Invitrogen

CD45RO Monoclonal Antibody (UCHL1), eFluor™ 450, eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3个已发表图片

6 篇参考文献

查看其他35个CD45RO抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD45RO Antibody in Flow Cytometry (Flow)

图: 1 / 5

CD45RO Antibody (48-0457-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Mouse IgG2a K Isotype Control eFluor® 450 (Product # 48-4724-82) (left) or Anti-Human CD45RO eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

48-0457-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

UCHL1

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10852567

产品详细信息

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

48-0457 was used in Flow cytometry/Cell sorting to determine the viral tropism of subtype C (C-HIV) Envelope (Env) clones for different CD4+ T cell subsets and whether tropism changes during acute to chronic disease progression.

Thu Aug 06 00:00:00 EDT 2020

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Thu Aug 06 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 48-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

48-0457 was used in Flow cytometry/Cell sorting to demonstrate that blocking PITPNM3 recognition of CCL18 could be an attractive immunotherapy aproach for treating breast cancer.

Sat Apr 01 00:00:00 EDT 2017

Chloroquine inhibits human CD4⁺ T-cell activation by AP-1 signaling modulation.

Scientific reports

Schmidt RL,Jutz S,Goldhahn K,Witzeneder N,Gerner MC,Trapin D,Greiner G,Hoermann G,Steiner G,Pickl WF,Burgmann H,Steinberger P,Ratzinger F,Schmetterer KG

48-0457 was used in Flow cytometry/Cell sorting to demonstrate that chloroquine has immunomodulatory effects on CD4(+) T-cells.

Tue Feb 07 00:00:00 EST 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

48-0457 was used in Flow cytometry/Cell sorting to investigate alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

Fri Apr 01 00:00:00 EDT 2016

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

Published figure using CD45RO monoclonal antibody (Product # 48-0457-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

查看更多收起

基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

查看更多收起

UniProt ID: (Human) P08575

查看更多收起

Entrez Gene ID: (Human) 5788

查看更多收起

功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

参与通路