Invitrogen

CD45RO Monoclonal Antibody (UCHL1), PE

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

10 篇参考文献

查看其他35个CD45RO抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

CD45RO Antibody in Flow Cytometry (Flow)

图: 1 / 4

CD45RO Antibody (MA1-10236) in Flow

Separation of human CD45R0 positive CD45RA negative lymphocytes (red-filled) from CD45R0 negative CD45RA positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral human whole blood stained using anti-human CD45R0 (UCHL1) PE Monoclonal antibody (Product # MA1-10236) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.

Product Image Gallery

产品信息

MA1-10236

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

20 µL/1x10^6 cells

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

UCHL1

抗原

Human IL-2 dependent T cells

查看免疫原

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

15mM sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11152908

产品详细信息

This antibody recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

靶标信息

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

A giant primary sclerosing lipogranuloma of the scrotum.

Turk patoloji dergisi

Mungan S,Karagüzel E,Turan C,Reıs A

MA1-10236 was used in immunohistochemistry to report on a case of giant primary sclerosing lipogranuloma of the scrotum

Tue Jul 08 00:00:00 EDT 2014

Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.

Journal of immunology (Baltimore, Md. : 1950)

Palin AC,Ramachandran V,Acharya S,Lewis DB

MA1-10236 was used in flow cytometry to test if impaired human neonatal CD4(+) T cell immunity is due to reduced signaling by naive CD4(+) T cells following engagement of the αβ-TCR/CD3 complex and CD28.

Fri Mar 15 00:00:00 EDT 2013

Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret.

Development (Cambridge, England)

Honma Y,Kawano M,Kohsaka S,Ogawa M

MA1-10236 was used in immunohistochemistry to study the role of Ret in the neurturin-mediated formation of precise axonal projections in the central nervous system

Thu Jul 01 00:00:00 EDT 2010

A patient with clinicopathologic features of small plaque parapsoriasis presenting later with plaque-stage mycosis fungoides: report of a case and comparative retrospective study of 27 cases of "nonprogressive" small plaque parapsoriasis.

Journal of the American Academy of Dermatology

Belousova IE,Vanecek T,Samtsov AV,Michal M,Kazakov DV

MA1-10236 was used in immunohistochemistry to report on a case of small plaque parapsoriasis with plaque-stage mycosis fungoides

Mon Sep 01 00:00:00 EDT 2008

Hyperplasia of hair follicles and other adnexal structures in cutaneous lymphoproliferative disorders: a study of 53 cases, including so-called pseudolymphomatous folliculitis and overt lymphomas.

The American journal of surgical pathology

Kazakov DV,Belousova IE,Kacerovska D,Sima R,Vanecek T,Vazmitel M,Pizinger K,Michal M

MA1-10236 was used in immunohistochemistry to study 53 cases of cutaneous lymphoproliferative disorders

Wed Oct 01 00:00:00 EDT 2008

Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

Cancer research

Carolan BJ,Heguy A,Harvey BG,Leopold PL,Ferris B,Crystal RG

MA1-10236 was used in immunocytochemistry to investigate the expression of carboxyl-terminal hydrolyase L1 gene in human airway epithelium of cigarette smokers

Wed Nov 15 00:00:00 EST 2006

Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Journal of cutaneous pathology

Boudova L,Kazakov DV,Jindra P,Sima R,Vanecek T,Kuntscher V,Vera V,Bouda J,Michal M

MA1-10236 was used in immunohistochemistry to report on a case of utaneous lymphoma of the head co-expressing CD56 and CD30

Tue Aug 01 00:00:00 EDT 2006

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA1-10236 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 EDT 2005

T-cell-rich B-cell lymphoma: a clinicopathologic study of 21 cases and comparison with 43 cases of diffuse large B-cell lymphoma.

Leukemia research

Aki H,Tuzuner N,Ongoren S,Baslar Z,Soysal T,Ferhanoglu B,Sahinler I,Aydin Y,Ulku B,Aktuglu G

MA1-10236 was used in immunohistochemistry to compare the clinocopathology of T-cell rich B-cell lymphoma and diffuse large B-cell lymphoma

Mon Mar 01 00:00:00 EST 2004

Gammadelta+ T cells preferentially respond to live rather than killed malaria parasites.

Infection and immunity

Waterfall M,Black A,Riley E

MA1-10236 was used in flow cytometry to study responses of peripheral blood T cells to malaria antigens.

Fri May 01 00:00:00 EDT 1998

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

查看更多收起

基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

查看更多收起

UniProt ID: (Human) P08575

查看更多收起

Entrez Gene ID: (Human) 5788

查看更多收起

功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

参与通路