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Invitrogen
CD48 Monoclonal Antibody (HM48-1), PerCP-eFluor™ 710, eBioscience™
产品信息
46-0481-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
HM48-1
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10853483
产品详细信息
Description: The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen; also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. Recent publications have reported differential expression of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors based on the expression of these SLAM family members. Hematopoietic stem cells (HSC) are highly purified as CD150(+)CD244(-)CD48(-) cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244(+)CD150(-)CD48(-) and the most restricted progenitors are CD48(+)CD244(+)CD150(-). CD48 plays a critical role in adhesion and T cell activation. In the mouse, the primary counter-receptors for CD48 are CD2 and CD244. HM48-1 is reported to modulate in vitro and in vivo CD48 functions including blocking the CD48/CD2 and CD48/CD244 interactions, inhibiting the proliferative response of mitogen-activated spleen cells, providing a costimulation signal for T cells activated in vitro through their TCR, and prolonging cardiac allograft survival in vivo.
Applications Reported: This HM48-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HM48-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: B-cell activation marker; BCM1 surface antigen; BLAST-1; CD48; CD48 antigen; CD48 antigen (B cell membrane protein); CD48d; HM48-1; MRC OX-45 surface antigen; sCD 48; sCD48; sgp-60; Signaling lymphocytic activation molecule 2; Signaling lymphocytic activation molecule 2 (SLAMF2); SLAM family member 2; SLAMF2; soluble CD 48; soluble CD48
基因别名: AI449234; AW610730; Bcm-1; BCM1; BLAST; BLAST-1; BLAST1; Cd48; MEM-102; Sgp-60; SLAMF2
UniProt ID: (Mouse) P18181
Entrez Gene ID: (Mouse) 12506
