Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), eBioscience™

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

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CD54 (ICAM-1) Antibody (14-0542-82) in Flow

Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD54 (ICAM-1) Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Cells in the lymphocyte gate were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF)

CD54 (ICAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD54 (ICAM-1) Antibody in Immunohistochemistry (IHC)

CD54 (ICAM-1) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

CD54 (ICAM-1) Antibody in Western Blot (WB)

CD54 (ICAM-1) Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

14-0542-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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免疫沉淀 (IP)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

Assay-Dependent

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioKAT-1 (KAT-1, KAT1)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_529543

产品详细信息

Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.

Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (Please use Functional Grade purified eBioKAT-1 (KAT-1, KAT1), Product # 16-0542, in functional assays).

Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Wed Nov 16 00:00:00 EST 2022

Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease.

Haematologica

Venugopal J,Wang J,Mawri J,Guo C,Eitzman D

14-0542-82 was used in Immunohistochemistry to test the effects of interleukin-1 receptor deletion in the pathophysiology of sickle cell disease-associated stroke.

Wed Sep 01 00:00:00 EDT 2021

Thrombin Inhibition Prevents Endothelial Dysfunction and Reverses 20-HETE Overproduction without Affecting Blood Pressure in Angiotensin II-Induced Hypertension in Mice.

International journal of molecular sciences

Kij A,Bar A,Przyborowski K,Proniewski B,Mateuszuk L,Jasztal A,Kieronska-Rudek A,Marczyk B,Matyjaszczyk-Gwarda K,Tworzydlo A,Enggaard C,Hansen PBL,Jensen B,Walczak M,Chlopicki S

14-0542-82 was used in Immunohistochemistry and Immunocytochemistry to assess the effects of the inhibition of thrombin activity by dabigatran on Ang II-induced hypertension and endothelial dysfunction in mice with a particular focus on NO- and 20-HETE-dependent pathways.

Thu Aug 12 00:00:00 EDT 2021

Thrombin Inhibition Prevents Endothelial Dysfunction and Reverses 20-HETE Overproduction without Affecting Blood Pressure in Angiotensin II-Induced Hypertension in Mice.

International journal of molecular sciences

Kij A,Bar A,Przyborowski K,Proniewski B,Mateuszuk L,Jasztal A,Kieronska-Rudek A,Marczyk B,Matyjaszczyk-Gwarda K,Tworzydlo A,Enggaard C,Hansen PBL,Jensen B,Walczak M,Chlopicki S

Thu Aug 12 00:00:00 EDT 2021

Effects of Grape Polyphenols on the Life Span and Neuroinflammatory Alterations Related to Neurodegenerative Parkinson Disease-Like Disturbances in Mice.

Molecules (Basel, Switzerland)

Tikhonova MA,Tikhonova NG,Tenditnik MV,Ovsyukova MV,Akopyan AA,Dubrovina NI,Amstislavskaya TG,Khlestkina EK

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Immunohistochemistry

Mon Nov 16 00:00:00 EST 2020

Dual specificity phosphatase DUSP6 promotes endothelial inflammation through inducible expression of ICAM-1.

The FEBS journal

Hsu SF,Lee YB,Lee YC,Chung AL,Apaya MK,Shyur LF,Cheng CF,Ho FM,Meng TC

14-0542-82 was used in Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation to suggest that manipulation of DUSP6 holds great potential for the treatment of acute inflammatory diseases.

Tue May 01 00:00:00 EDT 2018

Dual specificity phosphatase DUSP6 promotes endothelial inflammation through inducible expression of ICAM-1.

The FEBS journal

Hsu SF,Lee YB,Lee YC,Chung AL,Apaya MK,Shyur LF,Cheng CF,Ho FM,Meng TC

Tue May 01 00:00:00 EDT 2018

CD137 Plays Both Pathogenic and Protective Roles in Type 1 Diabetes Development in NOD Mice.

Journal of immunology (Baltimore, Md. : 1950)

Forsberg MH,Ciecko AE,Bednar KJ,Itoh A,Kachapati K,Ridgway WM,Chen YG

14-0542 was used in Immunohistochemistry-immunofluorescence to show that CD137 promotes the expansion and accumulation of Β cell-autoreactive CD8 T cells, and in the absence of CD137 or its interaction with CD137 ligand, T1D progression is suppressed.

Mon May 15 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Protective Effects of Emodin-Induced Neutrophil Apoptosis via the Ca²⁺-Caspase 12 Pathway against SIRS in Rats with Severe Acute Pancreatitis.

BioMed research international

Wang GJ,Wang Y,Teng YS,Sun FL,Xiang H,Liu JJ,Xia SL,Zhang GX,Chen HL,Shang D

14-0542 was used in Magnetic cell separation to explore the protective effects and underlying mechanisms of emodin against systemic inflammatory response syndrome in rats with severe acute pancreatitis.

Thu Jan 19 00:00:00 EST 2017

Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

Gastroenterology

Liu S,Li N,Yu X,Xiao X,Cheng K,Hu J,Wang J,Zhang D,Cheng S,Liu S

14-0542 was used in Flow cytometry/Cell sorting to demonstrate that the stem cell factor Nanog regulates expression of ICAM-1 in hepatocellular carcinoma cells.

Wed May 01 00:00:00 EDT 2013

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Immunoprecipitation

Mon Aug 01 00:00:00 EDT 2011

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Immunoprecipitation

Mon Aug 01 00:00:00 EDT 2011

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0542-82) in Immunoprecipitation

Mon Aug 01 00:00:00 EDT 2011

生物信息学

蛋白别名: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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功能

integrin binding protein complex binding

参与通路

CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), eBioscience™

CD54 (ICAM-1) Antibody (14-0542-82) in Flow

产品信息

14-0542-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

功能检测 (Functional)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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