Invitrogen

CD56 (NCAM) Monoclonal Antibody (CMSSB), Biotin, eBioscience™

CD56 (NCAM) Antibody in Flow Cytometry (Flow)

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CD56 (NCAM) Antibody (13-0567-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0036-42) and 0.25 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 0.25 µg of Anti-Human CD56 (NCAM) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

CD56 (NCAM) Antibody in Immunohistochemistry (IHC)

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产品信息

13-0567-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

CMSSB

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10596499

产品详细信息

Description: This CMSSB monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells. Staining with CMSSB does not block binding of MEM188 or CB56.

Applications Reported: This CMSSB antibody has been reported for use in flow cytometric analysis and immunohistology staining of paraffin embedded tissue sections.

Applications Tested: This CMSSB antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。