Invitrogen

CD56 (NCAM) Monoclonal Antibody (TULY56), eFluor™ 450, eBioscience™

CD56 (NCAM) Antibody in Flow Cytometry (Flow)

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CD56 (NCAM) Antibody (48-0566-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0036-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD56 (NCAM) eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

48-0566-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human, Non-human primate, Rhesus monkey

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

TULY56

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574021

产品详细信息

Description: This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells.

Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes. Additionally, TULY56 performs better after fixation and permeabilization than CMSSB.

The TULY56 monoclonal antibody crossreacts with Rhesus macaque.

Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TULY56 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Tue May 12 00:00:00 EDT 2020

Immune characterization of metastatic colorectal cancer patients post reovirus administration.

BMC cancer

Parakrama R,Fogel E,Chandy C,Augustine T,Coffey M,Tesfa L,Goel S,Maitra R

48-0566 was used in Flow cytometry/Cell sorting to evaluate the nature of immune response by determining the cytokine expression pattern in peripheral circulation along with the distribution of antigen presenting cells (APCs) and activated T lymphocytes.

Thu Jun 18 00:00:00 EDT 2020

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

48-0566 was used in Flow cytometry/Cell sorting to created a gene expression compendium across 24 distinct human and murine lung and lymph node mononuclear phagocyte (MP), along with human blood and murine spleen MPs, to serve as validation datasets.

Tue Nov 03 00:00:00 EST 2020

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Nature communications

Zou F,Lu L,Liu J,Xia B,Zhang W,Hu Q,Liu W,Zhang Y,Lin Y,Jing S,Huang M,Huang B,Liu B,Zhang H

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Wed Sep 11 00:00:00 EDT 2019

Disarming Staphylococcus aureus from destroying human cells by simultaneously neutralizing six cytotoxins with two human monoclonal antibodies.

Virulence

Rouha H,Weber S,Janesch P,Maierhofer B,Gross K,Dolezilkova I,Mirkina I,Visram ZC,Malafa S,Stulik L,Badarau A,Nagy E

48-0566 was used in Flow cytometry/Cell sorting to conclude that simultaneous inhibition of alpha-hemolysin and five leukocidins by ASN100 blocks cytolytic activity of S. aureus towards human target cells in vitro.

Mon Jan 01 00:00:00 EST 2018

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

48-0566 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 EDT 2017

Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers.

Journal of virology

Walker WE,Kurscheid S,Joshi S,Lopez CA,Goh G,Choi M,Barakat L,Francis J,Fisher A,Kozal M,Zapata H,Shaw A,Lifton R,Sutton RE,Fikrig E

Fri May 01 00:00:00 EDT 2015

Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria.

Science (New York, N.Y.)

Sonnenberg GF,Monticelli LA,Alenghat T,Fung TC,Hutnick NA,Kunisawa J,Shibata N,Grunberg S,Sinha R,Zahm AM,Tardif MR,Sathaliyawala T,Kubota M,Farber DL,Collman RG,Shaked A,Fouser LA,Weiner DB,Tessier PA,Friedman JR,Kiyono H,Bushman FD,Chang KM,Artis D

Published figure using CD56 (NCAM) monoclonal antibody (Product # 48-0566-42) in Flow Cytometry

Fri Jun 08 00:00:00 EDT 2012

生物信息学

蛋白别名: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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基因别名: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684, (Rhesus monkey) 693789

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功能

virus receptor activity Ras guanyl-nucleotide exchange factor activity

参与通路

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway