Invitrogen

CD57 Monoclonal Antibody (TB01 (TBO1)), PE-Cyanine7, eBioscience™

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CD57 Antibody (25-0577-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0036-42) and 1.0 µg of Mouse IgM Isotype Control APC (Product # 17-4752-80) (left) or 1.0 µg of Anti-Human CD57 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

25-0577-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

TB01 (TBO1)

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573354

产品详细信息

Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported: This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

25-0577-42 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

25-0577-42 was used in Flow Cytometry to conclude that early exposures predict the postnatal immune-microbiota axis trajectory, placing infants at later risk for respiratory morbidity in early childhood.

Fri Apr 15 00:00:00 EDT 2022

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

Fri Apr 15 00:00:00 EDT 2022

Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4⁺ T cell subsets in vivo.

Cell reports

Xie G,Luo X,Ma T,Frouard J,Neidleman J,Hoh R,Deeks SG,Greene WC,Roan NR

25-0577-42 was used in Flow Cytometry to provide an atlas of the phenotypes of in vivo and in vitro HIV-susceptible cells.

Tue Apr 27 00:00:00 EDT 2021

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Neidleman J,Luo X,Frouard J,Xie G,Hsiao F,Ma T,Morcilla V,Lee A,Telwatte S,Thomas R,Tamaki W,Wheeler B,Hoh R,Somsouk M,Vohra P,Milush J,James KS,Archin NM,Hunt PW,Deeks SG,Yukl SA,Palmer S,Greene WC,Roan NR

25-0577 was used in Flow cytometry/Cell sorting to describe a method that leverages high-dimensional phenotyping using CyTOF to trace latently infected cells reactivated ex vivo to their original pre-activation states.

Tue Sep 29 00:00:00 EDT 2020

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Tue Sep 29 00:00:00 EDT 2020

Function of multiple sclerosis-protective HLA class I alleles revealed by genome-wide protein-quantitative trait loci mapping of interferon signalling.

PLoS genetics

Lundtoft C,Pucholt P,Imgenberg-Kreuz J,Carlsson-Almlöf J,Eloranta ML,Syvänen AC,Nordmark G,Sandling JK,Kockum I,Olsson T,Rönnblom L,Hagberg N

25-0577 was used in Flow cytometry/Cell sorting to suggest that the MS-protective effects of HLA class I alleles are unrelated to their antigen-presenting function, and propose a previously unappreciated function of type I IFN signalling in B and T cells in MS immune-pathogenesis.

Thu Oct 01 00:00:00 EDT 2020

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 25-0577-42) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2017

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD57 monoclonal antibody (Product # 25-0577-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

生物信息学

蛋白别名: Beta-1,3-glucuronyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GlcUAT-P; Glucuronosyltransferase P; HNK-1; Leu-7; LEU7 antigen; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

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基因别名: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1

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UniProt ID: (Human) Q9P2W7

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Entrez Gene ID: (Human) 27087

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功能

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity metal ion binding glycosyltransferase

参与通路

carbohydrate metabolic process protein glycosylation glycosaminoglycan metabolic process chondroitin sulfate metabolic process chondroitin sulfate proteoglycan biosynthetic process