Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

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CD61 (Integrin beta 3) Antibody (14-0611-85) in Flow

Staining of C57Bl/6 bone marrow cells with 0.25 µg Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) Purified (filled histogram) followed byAnti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.

CD61 (Integrin beta 3) Antibody in Immunocytochemistry (ICC/IF)

CD61 (Integrin beta 3) Antibody in Immunohistochemistry (IHC)

CD61 (Integrin beta 3) Antibody in Western Blot (WB)

CD61 (Integrin beta 3) Antibody in Neutralization (Neu)

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产品信息

14-0611-85

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看9篇已发表文章 9篇已发表文章

中和实验 (Neu)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2C9.G3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_467312

产品详细信息

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, Product # 16-0611, in functional assays).

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

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基因别名: CD61; GP3A; INGRB3; Itgb3

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UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

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Entrez Gene ID: (Rat) 29302, (Mouse) 16416

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功能

fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin

参与通路

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

CD61 (Integrin beta 3) Antibody (14-0611-85) in Flow

产品信息

14-0611-85

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

中和实验 (Neu)

功能检测 (Functional)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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