Invitrogen

CD68 Monoclonal Antibody (FA-11), FITC

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CD68 Antibody (MA5-16676) in Flow

Journal of applied microbiology 2022 - 5 FIGURE L-norvaline combined with DOX affects the proliferation of breast cancer cells. (a) Identification of CD68 + CD206 positive cells by flow cytometry. (b) The gene expression of Arg-1 and IL-10 by quantitative real-time PCR . (c) The proliferative activity of cells after treatment with different concentrations of L-norvaline. (d) The proliferative activity of cells after treatment with DOX and differ... View More

CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

MA5-16676

应用

建议稀释比

已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

FA-11

抗原

purified Con A acceptor glycoprotein from P815 cell line

查看免疫原

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS with 1% BSA

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2538170

产品详细信息

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.

Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.

靶标信息

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The polarization of microglia and infiltrated macrophages in the injured mice spinal cords: a dynamic analysis.

PeerJ

Li JL,Fu GQ,Wang YY,Bian MM,Xu YM,Zhang L,Chen YQ,Zhang N,Ding SQ,Wang R,Fang R,Tang J,Hu JG,Lü HZ

MA5-16676 was used in Flow cytometry/Cell sorting to study if M1 cells in injured spinal cords were mainly derived from MG rather than infiltrating Mø.

Tue Feb 28 00:00:00 EST 2023

Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ₁ signaling pathway in diabetic atherosclerosis.

Molecular medicine (Cambridge, Mass.)

Zhao L,Li Y,Xu T,Lv Q,Bi X,Liu X,Fu G,Zou Y,Ge J,Chen Z,Zhang W

MA5-16676 was used in Immunohistochemistry (PFA fixed) to demonstrate that activation of the RAGE-TLR4-PKC1 signal pathway in a high-glucose and high-fat environment was involved in the maturation of dendritic cells and development of chronic inflammation.

Fri Jan 21 00:00:00 EST 2022

Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ₁ signaling pathway in diabetic atherosclerosis.

Molecular medicine (Cambridge, Mass.)

Zhao L,Li Y,Xu T,Lv Q,Bi X,Liu X,Fu G,Zou Y,Ge J,Chen Z,Zhang W

Fri Jan 21 00:00:00 EST 2022

L-norvaline affects the proliferation of breast cancer cells based on the microbiome and metabolome analysis.

Journal of applied microbiology

Zhu Q,Zai H,Zhang K,Zhang X,Luo N,Li X,Hu Y,Wu Y

Published figure using CD68 monoclonal antibody (Product # MA5-16676) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

The alveolar macrophage toponome of female SP-A knockout mice differs from that of males before and after SP-A1 rescue.

Scientific reports

Phelps DS,Chinchilli VM,Yang L,Shearer D,Weisz J,Zhang X,Floros J

MA5-16676 was used in flow cytometry to study the expression of alveolar macrophages in male and female mice lacking surfactant protein A.

Wed Mar 23 00:00:00 EDT 2022

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

MA5-16676 was used in Flow Cytometry to suggest that VX-765 can reduce neuroinflammation and improve nerve function recovery after spinal cord injury by inhibiting caspase-1/interleukin-1β/interleukin-18.

Wed Sep 01 00:00:00 EDT 2021

Single-Cell Transcriptome Profiles Reveal Fibrocytes as Potential Targets of Cell Therapies for Abdominal Aortic Aneurysm.

Frontiers in cardiovascular medicine

Li B,Song X,Guo W,Hou Y,Hu H,Ge W,Fan T,Han Z,Li Z,Yang P,Gao R,Zhao H,Wang J

MA1-82739 was used in Flow Cytometry to determine the roles of different vascular and immune cells in abdominal aortic aneurysm formation and pathogenesis.

Wed Dec 15 00:00:00 EST 2021

COX2 regulates senescence secretome composition and senescence surveillance through PGE₂.

Cell reports

Gonçalves S,Yin K,Ito Y,Chan A,Olan I,Gough S,Cassidy L,Serrao E,Smith S,Young A,Narita M,Hoare M

MA5-16676 was used in Flow Cytometry to show that, during RAS-induced senescence, COX2 is a critical regulator of the inflammatory senescence-associated secretory phenotype composition and senescence surveillance in vivo.

Tue Mar 16 00:00:00 EDT 2021

COX2 regulates senescence secretome composition and senescence surveillance through PGE₂.

Cell reports

Gonçalves S,Yin K,Ito Y,Chan A,Olan I,Gough S,Cassidy L,Serrao E,Smith S,Young A,Narita M,Hoare M

Tue Mar 16 00:00:00 EDT 2021

CRID3, a blocker of apoptosis associated speck like protein containing a card, ameliorates murine spinal cord injury by improving local immune microenvironment.

Journal of neuroinflammation

Chen YQ,Wang SN,Shi YJ,Chen J,Ding SQ,Tang J,Shen L,Wang R,Ding H,Hu JG,Lü HZ

MA5-16676 was used in Flow cytometry/Cell sorting to study the effect of CRID3 on the local microenvironment and the possible role in neuroprotection following spinal cord injury.