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Invitrogen
CD70 Monoclonal Antibody (113-16), eFluor™ 660, eBioscience™
图: 1 / 2
CD70 Antibody (50-0709-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 3 days with the Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) then stained with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Anti-Human CD70 eFluor® 660. Total viable cells were used for analysis.
产品信息
50-0709-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
113-16
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574171
产品详细信息
Description: The 113-16 monoclonal antibody reacts with human CD70, a type II transmembrane glycoprotein belonging to the TNF superfamily of proteins. CD70 is expressed weakly on macrophages and strongly on activated T, B, NK, and dendritic cells. Expression of CD70 can also be induced on smooth muscle and endothelial cells during inflammation. A ligand of CD27, CD70 plays a role in B cell growth and differentiation into plasma cells. Moreover, this receptor mediates Ig secretion of B cells by plasmacytoid DC. CD70 also plays a role in T cell proliferation and activation, as well as NK cell cytotoxicity. Studies have also shown that CD70 plays a role in regulating Th17 cells. In activated dendritic cells, CD70 helps activate CD8+ T cells and influences their development into memory T cells.
The 113-16 antibody has been reported to have antagonist activity.
Applications Reported: This 113-16 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 113-16 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD27 ligand; CD27-L; CD70; CD70 antigen; Ki-24 antigen; surface antigen CD70; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand 8A; Tumor necrosis factor ligand superfamily member 7
基因别名: CD27L; CD27LG; CD70; TNFSF7; TNLG8A
UniProt ID: (Human) P32970
Entrez Gene ID: (Human) 970
