Invitrogen

CD79a Monoclonal Antibody (HM47), PE, eBioscience™

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CD79a Antibody (12-0792-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) followed by intraceullular staining with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD79a PE (right) using IC Fixation and Permeabilization Buffers. Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0792-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Baboon, Bovine, Dog, Chicken, Chimpanzee, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rhesus monkey, Rat

已发表种属

Human, Mouse, Pig

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HM47

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2043848

产品详细信息

Description: The monoclonal antibody HM47 recognizes the cytoplasmic domain of CD79a, also known as mb-1. CD79a is a 47 kDa membrane glycoprotein that associates with CD79b to form the heterodimeric B cell receptor (BCR). This receptor is responsible for B cell signaling, resulting in activation, apoptosis or anergy. Expression of CD79a is found throughout development from the earliest pre-B cell to plasma cells. CD79 is expressed on B cell neoplasms and some non-B cell malignancies such as AML. Unlike other antibodies that can be masked in B cell neoplastic cells, HM47 is a reliable antibody for recognizing CD79a and, as such, is a good marker for B cells. Because several variants of CD79a exist, the exact location of the epitope may differ slightly. Nevertheless, in humans thie epitope is typically amino acids 208-222.

The HM47 antibody reacts to human, mouse, rat, dog, primates (rhesus, chimpanzee, macaque and baboon) pig, guinea rabbit, pig, horse, cow and chicken.

Applications Reported: This HM47 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This HM47 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD79a is a disulphide-linked heterodimer that includes B29 (CD79b) polypeptide. CD79a is a B lymphocyte antigen receptor with an antigen-specific surface component Ig (immunoglobulin) that associates with Ig-alpha and Ig-beta, necessary elements for the expression and function of the B-cell antigen receptor. CD79a first appears at pre-B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Diseases associated with CD79a include Agammaglobulinemia 3 and Agammaglobulinemia, Non-Bruton type.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium.

Stem cells international

Han B,Zhou L,Guan Q,da Roza G,Wang H,Du C

12-0792 was used in Flow cytometry/Cell sorting to show that upon expansion in a human protein medium, pMSCs shows a differential MSC marker expression profile from those of bone marrow or adipose tissue-derived MSCs and could maintain the multipotency.

Wed Sep 30 00:00:00 EDT 2020

The IgM receptor FcμR limits tonic BCR signaling by regulating expression of the IgM BCR.

Nature immunology

Nguyen TT,Kläsener K,Zürn C,Castillo PA,Brust-Mascher I,Imai DM,Bevins CL,Reardon C,Reth M,Baumgarth N

12-0792 was used in Flow cytometry/Cell sorting to study the expression and role of FcμR in the trans-Golgi network of developing B cells.

Wed Mar 01 00:00:00 EST 2017

Long-term spironolactone treatment reduces coronary TRPC expression, vasoconstriction, and atherosclerosis in metabolic syndrome pigs.

Basic research in cardiology

Li W,Chen X,Riley AM,Hiett SC,Temm CJ,Beli E,Long X,Chakraborty S,Alloosh M,White FA,Grant MB,Sturek M,Obukhov AG

12-0792 was used in Immunohistochemistry to study the role of TRPC1 and TRPC6 isoforms in coronary arteries of metabolic syndrome.

Fri Sep 01 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

12-0792 was used in Flow cytometry/Cell sorting to investigate the potential of hUCMSCs in improving ovarian function and withstanding ovarian senescence.

Thu Mar 09 00:00:00 EST 2017

生物信息学

蛋白别名: B-cell antigen receptor complex-associated protein alpha chain; B-cell antigen receptor complex-associated protein alpha chain-like; CD79; CD79-alpha protein; CD79a; CD79A antigen (immunoglobulin-associated alpha); CD79a molecule, immunoglobulin-associated alpha; Ig alpha; Ig-alpha; immunoglobulin-associated alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein

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基因别名: CD79A; EGK_10665; IG-alpha; IGA; Igalpha; Ly-54; Ly54; MB-1; MB1