Invitrogen

CD81 Monoclonal Antibody (M38)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2个已发表图片

4 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD81 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

CD81 Antibody (MA1-10290) in ICC/IF

Immunofluorescence analysis of CD81 antigen was performed using 70% confluent log phase MCF7 cells (a), MCF7 treated with Protein transport inhibitor (PTI) (1X for 4hrs) (b), and Hep G2 treated with Protein transport inhibitor (PTI) (1X for 4hrs) (c). The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with CD81 Monoclonal Antibody (M38) (Product # MA1-10290) at 1:100 dilution in 0.1% BSA, incubat... View More

Product Image Gallery

CD81 Antibody — Antibody clone (M38) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD81 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD81 antibody (M38). Yellow histogram represents staining with the isotype control. {KO}

产品信息

MA1-10290

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Cat, Human, Rabbit

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

M38

抗原

MOLT-4 (human T-ALL cell line)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11155175

产品详细信息

This antibody reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Western Blot: non-reducing conditions.

靶标信息

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Human Immunodeficiency Virus-Associated Exosomes Promote Kaposi's Sarcoma-Associated Herpesvirus Infection via the Epidermal Growth Factor Receptor.

Journal of virology

Chen L,Feng Z,Yuan G,Emerson CC,Stewart PL,Ye F,Jin G

MA1-10290 was used in Immunocytochemistry-immunoflourescence to reveal that the transmission Kaposi''s sarcoma-associated herpesvirus (KSHV) infection, which is promoted by the exosomes in the saliva of people living with human immunodeficiency virus (HIV), can reduced by the inhibition of epidermal growth factor receptor (EGFR).

Thu Apr 16 00:00:00 EDT 2020

Human Immunodeficiency Virus-Associated Exosomes Promote Kaposi's Sarcoma-Associated Herpesvirus Infection via the Epidermal Growth Factor Receptor.

Journal of virology

Chen L,Feng Z,Yuan G,Emerson CC,Stewart PL,Ye F,Jin G

Thu Apr 16 00:00:00 EDT 2020

Human Immunodeficiency Virus-Associated Exosomes Promote Kaposi's Sarcoma-Associated Herpesvirus Infection via the Epidermal Growth Factor Receptor.

Journal of virology

Chen L,Feng Z,Yuan G,Emerson CC,Stewart PL,Ye F,Jin G

Thu Apr 16 00:00:00 EDT 2020

Circulating exosomal microRNAs as novel potential detection biomarkers in pancreatic cancer.

Oncology letters

Wu L,Zhou WB,Zhou J,Wei Y,Wang HM,Liu XD,Chen XC,Wang W,Ye L,Yao LC,Chen QH,Tang ZG

MA1-10290 was used in Western Blotting to study the circulating ex-miRNA-21 and miRNA-210 as novel biomarkers for patients with pancreatic cancer.

Sat Aug 01 00:00:00 EDT 2020

Exosomes derived from HIV-1-infected cells promote growth and progression of cancer via HIV TAR RNA.

Nature communications

Chen L,Feng Z,Yue H,Bazdar D,Mbonye U,Zender C,Harding CV,Bruggeman L,Karn J,Sieg SF,Wang B,Jin G

Published figure using CD81 monoclonal antibody (Product # MA1-10290) in Western Blot

Fri Nov 02 00:00:00 EDT 2018

生物信息学

蛋白别名: 26 kDa cell surface protein TAPA-1; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28

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基因别名: CD81; CVID6; S5.7; TAPA1; TSPAN28

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UniProt ID: (Human) P60033

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Entrez Gene ID: (Human) 975

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功能

protein binding MHC class II protein complex binding transferrin receptor binding

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