Invitrogen

CD81 Recombinant Rabbit Monoclonal Antibody (SN206-01)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5个已发表图片

6 篇参考文献

查看其他47个CD81抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD81 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 16

CD81 Antibody (MA5-32333) in ICC/IF

Immunofluorescence analysis of CD81 antigen was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with CD81 Recombinant Rabbit Monoclonal Antibody (SN206-01) (Product # MA5-32333) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Al... View More

CD81 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD81 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD81 Recombinant Rabbit Monoclonal Antibody (SN206-01) (Product # MA5-32333), shows signal in MCF7 compared to Hep G2 showing no signal. Relative Expression. {RE}

产品信息

MA5-32333

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SN206-01

抗原

Synthetic peptide within Human TAPA1/CD81 aa 161-210

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809614

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway.

Analytical cellular pathology (Amsterdam)

Yu Z,Liu J,Fan Q,Yu J,Ren X,Wang X

Published figure using CD81 recombinant monoclonal antibody (Product # MA5-32333) in Western Blot

Mon Apr 04 00:00:00 EDT 2022

Neural stem cell-derived exosomes suppress neuronal cell apoptosis by activating autophagy via miR-374-5p/STK-4 axis in spinal cord injury.

Journal of musculoskeletal & neuronal interactions

Zhang L,Han P

MA5-32333 was used in Western Blotting to evaluate the roles of MicroRNAs (miRNAs) enclosed in the neuron-derived exosomes in the recovery of the spinal cord injury (SCI) and the mechanism.

Thu Sep 01 00:00:00 EDT 2022

Neural stem cell-derived exosomes suppress neuronal cell apoptosis by activating autophagy via miR-374-5p/STK-4 axis in spinal cord injury.

Journal of musculoskeletal & neuronal interactions

Zhang L,Han P

MA5-32333 was used in Western Blotting to evaluate the roles of MicroRNAs (miRNAs) enclosed in the neuron-derived exosomes in the recovery of the spinal cord injury (SCI) and the mechanism.

Thu Sep 01 00:00:00 EDT 2022

Cell death & disease

Zhang M,Gong W,Zhang D,Ji M,Chen B,Chen B,Li X,Zhou Y,Dong C,Wen G,Zhan X,Wu X,Cui L,Feng Y,Wang S,Yuan H,Xu E,Xia M,Verkhratsky A,Li B

Published figure using CD81 recombinant monoclonal antibody (Product # MA5-32333) in Western Blot

Mon Apr 25 00:00:00 EDT 2022

Platelet membrane and stem cell exosome hybrid enhances cellular uptake and targeting to heart injury.

Nano today

Hu S,Wang X,Li Z,Zhu D,Cores J,Wang Z,Li J,Mei X,Cheng X,Su T,Cheng K

MA5-32333 was used in Immunocytochemistry to provide proof-of-concept data and a universal approach to enhance the binding and accumulation of exosomes in injured tissues.

Sun Aug 01 00:00:00 EDT 2021

The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.

Journal of cellular and molecular medicine

Zhang Z,Ge L,Zhang S,Wang J,Jiang W,Xin Q,Luan Y

Published figure using CD81 recombinant monoclonal antibody (Product # MA5-32333) in Western Blot

Tue Dec 01 00:00:00 EST 2020

The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.

Journal of cellular and molecular medicine

Zhang Z,Ge L,Zhang S,Wang J,Jiang W,Xin Q,Luan Y

Published figure using CD81 recombinant monoclonal antibody (Product # MA5-32333) in Western Blot

Tue Dec 01 00:00:00 EST 2020

Macrophage secretion of miR-106b-5p causes renin-dependent hypertension.

Nature communications

Oh J,Matkovich SJ,Riek AE,Bindom SM,Shao JS,Head RD,Barve RA,Sands MS,Carmeliet G,Osei-Owusu P,Knutsen RH,Zhang H,Blumer KJ,Nichols CG,Mecham RP,Baldán Á,Benitez BA,Sequeira-Lopez ML,Gomez RA,Bernal-Mizrachi C

MA5-32333 was used in Western Blotting to confirm that macrophage miR-106b-5p secretion from impaired vitamin D receptor signalling causes inflammation-induced hypertension.

Wed Sep 23 00:00:00 EDT 2020

生物信息学

蛋白别名: 26 kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28

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基因别名: CD81; CVID6; S5.7; Tapa-1; TAPA1; TSPAN28

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UniProt ID: (Human) P60033, (Mouse) P35762

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Entrez Gene ID: (Human) 975, (Rat) 25621, (Mouse) 12520

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功能

protein binding MHC class II protein complex binding transferrin receptor binding

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