Invitrogen

CD86 (B7-2) Monoclonal Antibody (24F), FITC, eBioscience™

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

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CD86 (B7-2) Antibody (11-0860-82) in Flow

Wistar rat splenocytes were stimulated for 72 hours with LPS (Product # 00-4976-03). Cells were then stained with 0.25 µg of Mouse IgG1 kappa Isotype Control, FITC (Product # 11-4714-82) (blue histogram) or 0.25 µg of CD86 Monoclonal Antibody, FITC (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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产品信息

11-0860-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

24F

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465145

产品详细信息

Description: The 24F monoclonal antibody reacts with rat CD86, an ~90 kDa surface receptor, also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed by germinal center B cells and at low levels on macrophages, dendritic cells and non-lymphoid cells in the thymus. Its expression is upregulated on B cells through a variety of surface stimuli including LPS. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported: The 24F antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 24F antibody has been tested by flow cytometric analysis of resting and activated rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Acinar Cell-Derived Extracellular Vesicle MiRNA-183-5p Aggravates Acute Pancreatitis by Promoting M1 Macrophage Polarization Through Downregulation of FoxO1.

Frontiers in immunology

Tang DS,Cao F,Yan CS,Cui JT,Guo XY,Cheng L,Li L,Li YL,Ma JM,Fang K,Gao L,Ren NS,Sun B,Wang G,Ji L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0860-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2022

Morphine-induced changes in the function of microglia and macrophages after acute spinal cord injury.

BMC neuroscience

Terminel MN,Bassil C,Rau J,Trevino A,Ruiz C,Alaniz R,Hook MA

11-0860-82 was used in Flow cytometry/Cell sorting to suggest that morphine administered immediately after an SCI changes the innate immune response by increasing the number of immune cells and altering neuropeptide synthesis by these cells.

Mon Oct 10 00:00:00 EDT 2022

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0860-82) in Flow Cytometry

Fri Apr 29 00:00:00 EDT 2022

Dental Follicle Stem Cells Ameliorate Lipopolysaccharide-Induced Inflammation by Secreting TGF-β3 and TSP-1 to Elicit Macrophage M2 Polarization.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Chen X,Yang B,Tian J,Hong H,Du Y,Li K,Li X,Wang N,Yu X,Wei X

11-0860-82 was used in Flow cytometry/Cell sorting to support the hypothesis that DFSCs are promising for MSC-based immunotherapy.

Fri Jan 25 00:00:00 EST 2019

Culling of APCs by inflammatory cell death pathways restricts TIM3 and PD-1 expression and promotes the survival of primed CD8 T cells.

Cell death and differentiation

Patel R,Kim K,Shutinoski B,Wachholz K,Krishnan L,Sad S

11-0860 was used in Flow cytometry/Cell sorting to evaluate the impact of premature cell death of antigen-presenting cells by Caspase-1- and RipK3-signalling pathways on CD8+ T-cell priming during infection of mice with Salmonella typhimurium.

Wed Nov 01 00:00:00 EDT 2017

Amelioration of collagen-induced arthritis using antigen-loaded dendritic cells modified with NF-κB decoy oligodeoxynucleotides.

Drug design, development and therapy

Jiang H,Hu H,Zhang Y,Yue P,Ning L,Zhou Y,Shi P,Yuan R

Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0860-82) in Flow Cytometry

Mon Jul 23 00:00:00 EDT 2018

Interleukin-4 affects the mature phenotype and function of rat bone marrow-derived dendritic cells.

Molecular medicine reports

Wang S,Sun X,Zhou H,Zhu Z,Zhao W,Zhu C

11-0860-82 was used in Flow cytometry/Cell sorting to demonstrate that IL‚4 influences DC morphology and immune function, and that this process may be associated with the activation of STAT6.

Wed Jul 01 00:00:00 EDT 2015

生物信息学

蛋白别名: B lymphocyte activation antigen B72; CD28 antigen ligand 2; cd86 antigen; Early T-cell co-stimulatory molecule 1; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86

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基因别名: B7-2

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Entrez Gene ID: (Rat) 56822

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功能

receptor binding immunoglobulin receptor superfamily

参与通路