Invitrogen

CD90 (Thy-1) Monoclonal Antibody (G7), Functional Grade, eBioscience™

CD90 (Thy-1) Antibody in Flow Cytometry (Flow)

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CD90 (Thy-1) Antibody (16-0901-85) in Flow

Surface staining of mouse splenocytes with Anti-Mouse CD90 (Thy-1) Purified followed by Anti-Rat IgG FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD90 (Thy-1) Antibody in Immunohistochemistry (IHC)

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产品信息

16-0901-85

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

0.125 µg/test

功能检测 (Functional)

Assay-Dependent

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2c, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469032

产品详细信息

Description: The G7 monoclonal antibody reacts with mouse CD90 also known as Thy-1, a GPI-linked membrane molecule. CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells as well as neurons. CD90 is involved in regulation of adhesion and signal transduction by T cells. G7 is reported to induce TCR-mediated proliferation of T cell and apoptosis of thymocytes.

Applications Reported: The G7 antibody has been reported for use in flow cytometric analysis. G7 has also been reported in functional studies.

Applications Tested: The G7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

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