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产品信息
11-0982-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Rhesus monkey
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
5E5
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572465
产品详细信息
Description: The monoclonal antibody 5E5 recognizes human CD98hc protein also called CD98 heavy chain, FRP-1 (fusion regulatory protein-1 ) or 4F2. CD98 is a disulfide-linked complex composed of a heavy chain (a Type II membrane protein) and a light chain of which there are more than six different chains (LAT1, LAT2, y+LAT1, y+LAT2, Asc-1, xCT). CD98 can associate directly or indirectly with beta 1 integrins, EpCAM and CD147 (EMMPRIN). The function of CD98 is thought to involve amino acid transport, cell fusion concomitantly with increased plasma membrane expression and integrin mediated adhesion. Expression of CD98 is broad including non-hematopoietic cells. In peripheral blood cells, monocytes expression is high while lymphocytes have intermediate levels and granulocytes express very little.
Applications Reported: This 5E5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5E5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with beta1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: 4F2 cell-surface antigen heavy chain; 4F2 heavy chain antigen; 4F2hc; Amino acid transporter heavy chain SLC3A2; antigen defined by monoclonal antibody 4F2, heavy chain; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43; CD98; CD98 heavy chain; Lymphocyte activation antigen 4F2 large subunit; monoclonal antibody 44D7; solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2; solute carrier family 3 (amino acid transporter heavy chain), member 2; Solute carrier family 3 member 2
基因别名: 4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE; SLC3A2
UniProt ID: (Human) P08195
Entrez Gene ID: (Human) 6520
