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Invitrogen
CDK1 Recombinant Rabbit Monoclonal Antibody (SM01-44)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 11
CDK1 Antibody (MA5-32055) in ICC/IF
Immunofluorescence analysis of CDK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CDK1 Recombinant Rabbit Monoclonal Antibody (SM01-44) (Product # MA5-32055) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
产品信息
MA5-32055
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SM01-44
抗原
Synthetic peptide within Human CDK1 aa 228-277
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809349
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ABL1; CDK1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase
基因别名:
CDC2; CDC28A; Cdc2a; CDK1; CDKN1; p34
UniProt ID: (Human) P06493, (Rat) P39951, (Mouse) P11440
Entrez Gene ID: (Human) 983, (Rat) 54237, (Mouse) 12534
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
cyclin binding
Hsp70 protein binding
histone kinase activity
kinase activity
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
activation of MAPK activity
microtubule cytoskeleton organization
DNA replication
DNA repair
protein complex assembly
apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic nuclear division
mitotic nuclear envelope disassembly
mitotic G2 DNA damage checkpoint
centrosome cycle
pronuclear fusion
cell aging
response to toxic substance
positive regulation of gene expression
regulation of Schwann cell differentiation
response to organic cyclic compound
response to amine
response to activity
cell migration
histone phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
chromosome condensation
epithelial cell differentiation
organ regeneration
anaphase-promoting complex-dependent catabolic process
positive regulation of protein import into nucleus, translocation
protein localization to kinetochore
response to drug
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
response to ethanol
positive regulation of DNA replication
positive regulation of mitotic cell cycle
regulation of embryonic development
response to cadmium ion
response to copper ion
response to axon injury
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
cellular response to hydrogen peroxide
Golgi disassembly
positive regulation of protein localization to nucleus
mitotic cell cycle
protein phosphorylation
response to organonitrogen compound
response to hydrogen peroxide
cell cycle
phosphorylation
