Invitrogen

CDK5 Recombinant Rabbit Monoclonal Antibody (23GB1425)

查看其他22个CDK5抗体

抗体检测数据 (3)

CDK5 Antibody in Immunocytochemistry (ICC/IF)

CDK5 Antibody in Immunocytochemistry (ICC/IF)

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CDK5 Antibody (MA5-53156) in ICC/IF

Immunocytochemistry analysis of CDK5 in HepG2 cells. Nuclei were stained blue with DAPI; CDK5 recombinant monoclonal antibody (Product # MA5-53156) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

CDK5 Antibody in Immunocytochemistry (ICC/IF)

CDK5 Antibody in Western Blot (WB)

CDK5 Antibody in Flow Cytometry (Flow)

Product Image Gallery

CDK5 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CDK5 in HepG2 cells. Nuclei were stained blue with DAPI; CDK5 recombinant monoclonal antibody (Product # MA5-53156) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

CDK5 Antibody in Western Blot (WB) — Western Blot analysis of CDK5. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDK5 recombinant monoclonal antibody (Product # MA5-53156) with a dilution of 1:10,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

CDK5 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CDK5 in HepG2 cells using CDK5 recombinant monoclonal antibody (Product # MA5-53156) at a dilution of 1:2,000. Green, isotype control; red, CDK5.

产品信息

MA5-53156

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

-

免疫细胞化学 (ICC/IF)

1:1,000

-

流式细胞分析 (Flow)

1:2,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB1425

抗原

A synthesized peptide derived from human Cdk5 (250-292AA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5. CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CDK5-SV; Cell division protein kinase 5; CR6 protein kinase; Cyclin-dependent kinase 5; Cyclin-dependent-like kinase 5; protein kinase CDK5 splicing; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit

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基因别名: AW048668; CDK5; CDKN5; Crk6; LIS7; PSSALRE

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UniProt ID: (Human) Q00535, (Mouse) P49615, (Rat) Q03114

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Entrez Gene ID: (Human) 1020, (Mouse) 12568, (Rat) 140908

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p53 binding protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity ErbB-2 class receptor binding protein binding ATP binding cytoskeletal protein binding kinase activity protein kinase binding acetylcholine receptor activator activity ErbB-3 class receptor binding ephrin receptor binding tau-protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

参与通路

neuron migration synaptic transmission, dopaminergic intracellular protein transport nucleocytoplasmic transport cell cycle cell-matrix adhesion chemical synaptic transmission synapse assembly skeletal muscle tissue development motor neuron axon guidance cell proliferation visual learning response to wounding Schwann cell development synaptic vesicle exocytosis regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cerebellar cortex formation hippocampus development layer formation in cerebral cortex central nervous system neuron development corpus callosum development neuron differentiation regulation of cell migration negative regulation of axon extension cortical actin cytoskeleton organization neuron projection development negative regulation of protein ubiquitination negative regulation of synaptic plasticity positive regulation of protein binding receptor catabolic process serine phosphorylation of STAT3 protein synaptic transmission, glutamatergic protein localization to synapse regulation of apoptotic process receptor clustering positive regulation of neuron apoptotic process negative regulation of cell cycle positive regulation of protein kinase activity negative regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of calcium ion-dependent exocytosis protein autophosphorylation negative regulation of protein export from nucleus behavioral response to cocaine regulation of synaptic plasticity synaptic vesicle endocytosis rhythmic process axon extension oligodendrocyte differentiation dendrite morphogenesis cell division neuron apoptotic process excitatory postsynaptic potential regulation of dendritic spine morphogenesis calcium ion import regulation of cell cycle arrest positive regulation of protein targeting to membrane negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of synaptic vesicle recycling positive regulation of actin cytoskeleton reorganization positive regulation of receptor activity protein phosphorylation exocytosis apoptotic process nervous system development axonogenesis associative learning cell migration telencephalon development cerebellum development cerebellar cortex development cerebral cortex development forebrain development response to cocaine regulated exocytosis neuron projection morphogenesis regulation of cell cycle regulation of postsynaptic membrane potential central nervous system development