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CHKA Antibody (PA5-109529) in WB
Western blot analysis of CHKA in extracts of various cell lines. Samples were incubated with CHKA Polyclonal antibody (Product # PA5-109529) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-109529
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CHKA (NP_001268.2).
偶联物
Unconjugated
形式
Liquid
浓度
1.41 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2854940
产品详细信息
Immunogen sequence: MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQMRSCNKE GSEQAQKENE
靶标信息
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase alpha is the initial enzyme in the sequence and may play a regulatory role. This protein also catalyzes the phosphorylation of ethanolamine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CHETK-alpha; Choline kinase alpha; choline kinase R; choline/ethanolamine kinase alpha; CK; EK; Ethanolamine kinase
基因别名: Chetk-alpha; CHK; CHKA; ChoK; CK; CK-R; CK/EK-alpha; CKI; Ckr; EK; EtnK-alpha
UniProt ID: (Human) P35790, (Mouse) Q8BPL3, (Rat) Q01134
Entrez Gene ID: (Human) 1119, (Mouse) 12660, (Rat) 29194
choline kinase activity
cholinesterase activity
ethanolamine kinase activity
signal transducer activity
ATP binding
drug binding
choline binding
protein homodimerization activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
kinase
ethanolamine metabolic process
lipid metabolic process
phosphatidylethanolamine biosynthetic process
phosphatidylcholine biosynthetic process
CDP-choline pathway
lipid transport
signal transduction
response to toxic substance
phosphorylation
choline metabolic process
response to 3-methylcholanthrene
phospholipid biosynthetic process
