Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 1
CHRM3 Antibody (PA5-89906) in WB
Western blot analysis of CHRM3 in extracts of various cell lines. Samples were incubated with CHRM3 Polyclonal antibody (Product # PA5-89906) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-89906
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 390-475 of human CHRM3 (NP_0007311)
偶联物
Unconjugated
形式
Liquid
浓度
0.57 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2805833
产品详细信息
Immunogen sequence: QVPEEELGMV DLERKADKLQ AQKSVDDGGS FPKSFSKLPI QLESAVDTAK TSDVNSSVGK STATLPLSFK EATLAKRFAL KTRSQI; Positive Samples: SH-SY5Y, U-251MG, U-87MG; Cellular Location: Basolateral cell membrane, Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
靶标信息
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: acetylcholine receptor; acetylcholine receptor, muscarinic 3; cholinergic receptor; cholinergic receptor, muscarinic 3; m3 muscarinic receptor; Muscarinic acetylcholine receptor M3
基因别名: CHRM3; EGBRS; HM3; PBS
UniProt ID: (Human) P20309
Entrez Gene ID: (Human) 1131
regulation of vascular smooth muscle contraction
cellular protein modification process
smooth muscle contraction
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway
G-protein coupled acetylcholine receptor signaling pathway
synaptic transmission, cholinergic
nervous system development
cell proliferation
positive regulation of smooth muscle contraction
saliva secretion
