Proteintech

AHR Monoclonal Antibody (2D1F9), CoraLite® Plus 488

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AHR Antibody (CL488-67785) in Flow

1X10^6 HepG2 cells were intracellularly stained with 0.8 µg CoraLite® Plus 488 Anti-Human AHR (CL488-67785, Clone:2D1F9) (red), or 0.8 µg Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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产品信息

CL488-67785

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.8 µg/1x10^6 cell suspension

产品规格

种属反应

Human, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

2D1F9

抗原

Recombinant protein

查看免疫原

偶联物

CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488

激发/发射光谱

493/522 nm

形式

Liquid

纯化类型

Protein A

保存液

PBS with 50% glycerol, 0.5% BSA

内含物

0.05% ProClin 300

保存条件

-20° C, store in dark

运输条件

Wet ice

产品详细信息

Immunogen sequence: PIPAEGIKSN PSKRHRDRLN TELDRLASLL PFPQDVINKL DKLSVLRLSV SYLRAKSFFD VALKSSPTER NGGQDNCRAA NFREGLNLQE GEFLLQALNG FVLVVTTDAL VFYASSTIQD YLGFQQSDVI HQSVYELIHT EDRAEFQRQL HWALNPSQCT ESGQGIEEAT GLPQTVVCYN PDQIPPENSP LMERCFICRL RCLLDNSSGF LAMNFQGKLK YLHGQKKKGK DGSILPPQLA LFAIATPLQP PSILEIRTKN FIFRTKHKLD FTPIGC

靶标信息

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。