Invitrogen

CLUH Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 篇参考文献

查看其他10个CLUH抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

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CLUH Antibody (PA5-71324) in WB

Western blot analysis of CLUH on human MDA-MB-435s cells. The sample was probed with a CLUH polyclonal antibody (Product # PA5-71324) using a primary antibody dilution of 1.0 µg/mL.

Product Image Gallery

CLUH Antibody — Figure 8. Knockdown of the clustered mitochondria homolog (CLUH) elicits perinuclear mitochondrial redistribution, elevated mitochondrial ROS generation, and enhances RSV virus production. ( A-E ) A549 cells were pretreated (48 hr) with siRNA (50 nM) for clustered mitochondria homolog ( CLUH ), or scrambled siRNA control (scr) followed by infection with RSV (MOI 1) for another 24 hr. ( A ) Immunoblot analysis for CLUH , or the control alpha-tubulin, as indicated (40 ug cell lysate protein/lane). ( B and C ) Immunostaining and R 90% analysis were as per ( Figure 1C and D ). In all panels, scale bar = 10 um. Results represent the mean +- SEM for n = 3 independent experiments, where each experiment analysed 25-30 cells per sample; ***p<0.001. ( D ) Cells were stained with Mitotracker Deep Red and the mitochondria-specific ROS probe FRR2 as per Figure 7A . The ratiometric output images of I (Ex514) /I (Ex488) were calculated as per Figure 7A . ( E ) FRR2 (I (Ex514) /I (Ex488) ) was calculated for the mitochondrial regions defined by Mitotracker Deep Red staining in the I (Ex514) /I (Ex488) images such as those in D ) using a custom CellProfiler pipeline as per Figure 7B . Results represent the mean +- SEM for n = 3 independent experiments, where each experiment analysed 25-30 cells per sample, ***p<0.001, **p<0.01. Figure 8--figure supplement 1. Microtubule/dynein-dependent mitochondrial redistribution favours RSV virus production. Infectious virus (plaque forming units pfu ml -1 {KD}

产品信息

PA5-71324

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1.0 µg/mL

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

synthetic peptide directed towards the C-terminal region of human CLUH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2690757

靶标信息

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.

eLife

Hu M,Schulze KE,Ghildyal R,Henstridge DC,Kolanowski JL,New EJ,Hong Y,Hsu AC,Hansbro PM,Wark PA,Bogoyevitch MA,Jans DA

PA5-71324 was used in Western Blot to establish respiratory syncytial viruses unique ability to co-opt host cell mitochondria to facilitate viral infection, revealing the RSV-mitochondrial interface for the first time as a viable target for therapeutic intervention.