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CNOT1 Antibody (PA5-99966) in IHC (P)
Immunohistochemistry analysis of CNOT1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CNOT1 polyclonal antibody (Product # PA5-99966) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
产品信息
PA5-99966
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CNOT1(Accession A5YKK6), corresponding to amino acid residues S785-E835.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818899
产品详细信息
Antibody detects endogenous levels of total CNOT1.
靶标信息
Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: adrenal gland protein AD-005; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 1; CCR4-NOT transcription complex, subunit 1; Negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1H
基因别名: 6030411K04Rik; AA815922; AD-005; CDC39; CNOT1; D830048B13; KIAA1007; NOT1; NOT1H; RGD1308009
UniProt ID: (Human) A5YKK6, (Mouse) Q6ZQ08
Entrez Gene ID: (Human) 23019, (Rat) 291841, (Mouse) 234594
poly(A)-specific ribonuclease activity
protein binding
protein domain specific binding
estrogen receptor binding
protein complex scaffold
retinoic acid receptor binding
poly(A) RNA binding
armadillo repeat domain binding
molecular_function
mRNA polyadenylation factor
RNA metabolism protein
RNA processing factor
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
nuclear-transcribed mRNA poly(A) tail shortening
transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
multicellular organism development
positive regulation of cytoplasmic mRNA processing body assembly
negative regulation of translation
negative regulation of intracellular estrogen receptor signaling pathway
gene silencing by miRNA
negative regulation of retinoic acid receptor signaling pathway
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
regulation of stem cell population maintenance
trophectodermal cell differentiation
biological_process
regulation of transcription, DNA-templated
regulation of translation
gene silencing by RNA
