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Invitrogen
COL4A2 Polyclonal Antibody
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COL4A2 Antibody (PA5-79058) in IHC (P)
Immunohistochemistry (Paraffin) analysis of COL4A2 in paraffin-embedded section of human breast cancer tissue using COL4A2 Polyclonal Antibody (Product # PA5-79058). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnig... View More
Product Details
PA5-79058
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Canstatin (1697-1712aa LIRTHISRCQVCMKNL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue. IHC: human breast cancer tissue, human breast cancer tissue, human placenta tissue.
Target Information
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Collagen alpha-2(IV) chain
Gene Aliases: COL4A2; ICH; POREN2
UniProt ID: (Human) P08572
Entrez Gene ID: (Human) 1284
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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