Invitrogen

COX2 Monoclonal Antibody (COX 229), Alexa Fluor™ 647, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他37个COX2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

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COX2 Antibody (51-9725-82) in Flow

Normal human peripheral blood cells were stimulated for 5 hours with LPS (Product # 00-4976-03) and then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD14 Monoclonal Antibody, PE (Product # 12-0149-42) and 0.125 µg Mouse IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4714-0) (left) or COX2 Monoclonal Antibody, Alexa Fluor 647 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

Product Image Gallery

COX2 Antibody — Normal human peripheral blood cells were unstimulated (orange histogram) or stimulated with LPS (Product # 00-4976-03). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with Mouse IgG1 kappa Isotype Control (blue histogram) or COX2 Monoclonal Antibody (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18) were used for analysis (Product #65-0863-18). {RE}

产品信息

51-9725-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

COX 229

抗原

Synthetic peptide from human COX2

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2802310

产品详细信息

Description: This COX 229 monoclonal antibody recognizes human COX2 also known as PTGS2. This antibody has been reported to cross-react with the mouse and rat COX2. For optimal performance we recommend using this antibody with the Foxp3 / Transcription Factor Staining Buffer Set and protocol. This antibody is predicted to cross-react with the mouse and rat homologue.

Applications Reported: This COX 229 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This COX 229 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

靶标信息

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

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基因别名: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2; PTGS2

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UniProt ID: (Human) P35354

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Entrez Gene ID: (Human) 5743

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功能

peroxidase activity prostaglandin-endoperoxide synthase activity protein binding lipid binding enzyme binding heme binding protein homodimerization activity metal ion binding arachidonate 15-lipoxygenase activity oxygenase

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