Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
CRALBP Antibody (MA5-50818) in ICC/IF
Immunocytochemistry analysis of CRALBP in B16F1 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with CRALBP recombinant monoclonal antibody (Product # MA5-50818) with a dilution of 1:50 in 2% negative goat serum overnight at 4 . Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody... View More
产品信息
MA5-50818
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE35-09
抗原
Synthetic peptide within Human CRALBP aa 26-75/317.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3092420
产品详细信息
Positive control: Rat eyeball tissue lysate, mouse eyeball tissue lysate, B16F1.
Predicted band size: 36.5 kDa
Subcellular Location: Cytoplasm.
靶标信息
Cellular retinaldehyde-binding protein (CRALBP) plays an important role in the regeneration of 11-cis-retinal for use in rod visual pigments such as opsin and rhodopsin. Once 11-cis-retinal is photoisomerized in the rod outer segment, it is converted to all-trans-retinal and further modified into all-trans-retinol. All-trans-retinol then diffuses into the retinal pigment epithelium (RPE) to be converted back to 11-cis-retinol and further oxidized into 11-cis-retinal (both by CRALBP). Genetic mutations involving CRALBP's lack of function have been linked to visual disease such as bothnia dystrophy, retinitis punctata albescens, retina pigmentosa, and Newfoundland rod-cone dystrophy. The presence of CRALBP serves as a marker for RPE and Muller glial cells of the retina.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Cellular retinaldehyde-binding protein; Retinaldehyde-binding protein 1
基因别名: 3110056M11Rik; CRALBP; Rlbp1
UniProt ID: (Mouse) Q9Z275
Entrez Gene ID: (Mouse) 19771, (Rat) 293049
