Invitrogen

CRBN Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他8个CRBN抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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CRBN Antibody (PA5-61122) in WB

Western blot analysis of CRBN in mouse cell line NIH-3T3 and rat cell line NBT-II using a CRBN Polyclonal Antibody (Product # PA5-61122).

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CRBN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with, control siRNA, CRBN siRNA probe #1, and a decrease in signal intensity was observed in Western Blot application using Anti-CRBN Polyclonal Antibody (Product # PA5-61122) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

产品信息

PA5-61122

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human CRBN. Recombinant protein control fragment (Product #RP-96271).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2640143

产品详细信息

Immunogen sequence: EVEDQDSKEA KKPNIINFDT SLPTSHTYLG ADMEEFHGRT LHDDDSCQVI PVLPQVMMIL IPGQTLPLQL FHPQEVSMVR NLIQKDRTFA VLAYSNVQER E

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 97%.

靶标信息

CRBN, a member of the Lon protease protein family, plays a role in brain development. It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。