Invitrogen

CRBN Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他8个CRBN抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (1)

CRBN Antibody in Immunocytochemistry (ICC/IF)

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CRBN Antibody (PA5-98708) in ICC/IF

Immunofluorescence analysis of Protein cereblon was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CRBN Polyclonal Antibody (Product # PA5-98708) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 d... View More

CRBN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CRBN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Protein cereblon siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CRBN Polyclonal Antibody (Product # PA5-98708). siRNA knockdown was observed for only 55 kDa isoform of CRBN. {KD}

产品信息

PA5-98708

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:100

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Protein cereblon protein (1-280AA)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.59 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_2813321

靶标信息

CRBN, a member of the Lon protease protein family, plays a role in brain development. It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。