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Application
FIGURE: 1 / 2
CRHR1 Antibody (PA5-18625) in ICC/IF
Immunocytochemistry analysis of CRHR1 using CRHR1 Polyclonal Antibody (Product # PA5-18625) in paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18625
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (KLYYDNEKCWFGKR) corresponding to the internal amino acids of CRHR1 (aa 279-292).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10983269
Product Specific Information
This antibody is predicted to react with bovine, canine, human, ovine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1,000.
Target Information
Corticotropin-releasing factor receptor 1 (CRFR1/CRHR1), a Corticotropin-Releasing Factor Receptor, is involved in mediating the stress response and anxiety-related behaviors. Mutations of the receptor reduce anxiety-related behaviors and may be involved in the pathogenesis of Cushing disease. Multiple isoforms of CRFR1 are produced by alternative splicing. CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Corticotropin-releasing factor receptor 1; corticotropin-releasing factor type 1 receptor; Corticotropin-releasing hormone receptor 1; CRF 1 receptor; Crf receptor 1; Crf type 1; CRF-R-1; CRF-R1; CRF-R1alpha; Crf1 receptor; CRFR-1; CRH receptor 1; CRH receptor subtype-1 isoform-3; CRH-R-1; CRH-R1; CRH-R1h; CRHR1f; seven transmembrane helix receptor
Gene Aliases: CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1; CRHR1L
UniProt ID: (Human) P34998, (Mouse) P35347
Entrez Gene ID: (Human) 1394, (Mouse) 12921
protein binding
corticotrophin-releasing factor receptor activity
G-protein alpha-subunit binding
signal transducer activity
transmembrane signaling receptor activity
G-protein coupled receptor activity
protein complex binding
peptide binding
corticotropin-releasing hormone receptor activity
corticotropin-releasing hormone binding
transmembrane signal receptor
immune response
cell surface receptor signaling pathway
activation of adenylate cyclase activity
female pregnancy
parturition
regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
corticotropin secretion
cellular response to corticotropin-releasing hormone stimulus
negative regulation of voltage-gated calcium channel activity
regulation of corticosterone secretion
response to hypoxia
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
neuropeptide signaling pathway
memory
feeding behavior
visual learning
hormone-mediated signaling pathway
adrenal gland development
positive regulation of cAMP biosynthetic process
epithelial cell differentiation
negative regulation of epinephrine secretion
locomotory exploration behavior
fear response
positive regulation of mast cell degranulation
behavioral response to cocaine
behavioral response to ethanol
regulation of synaptic plasticity
behavioral response to pain
response to electrical stimulus
general adaptation syndrome, behavioral process
long-term synaptic potentiation
negative regulation of neuron death
negative regulation of feeding behavior
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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