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CRM1 Antibody (PA5-87417) in WB
Western blot analysis of CRM1 in extracts of various cell lines. Samples were incubated with CRM1 Polyclonal antibody (Product # PA5-87417) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
PA5-87417
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 964-1064 of human CRM1/CRM1/XPO1 (NP_0033911)
偶联物
形式
Liquid
浓度
1.85 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804132
产品详细信息
Positive Samples: U-87MG, B-cell, Mouse liver; Cellular Location: Cajal body, Cytoplasm, Nucleus, nucleolus, nucleoplasm
Immunogen sequence: PGNPVNNQIF LQEYVANLLK SAFPHLQDAQ VKLFVTGLFS LNQDIPAFKE HLRDFLVQIK EFAGEDTSDL FLEEREIALR QADEEKHKRQ MSVPGIFNPH E
靶标信息
Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: chromosome region maintenance 1 homolog; Chromosome region maintenance 1 protein homolog; Exp1; exportin 1 (CRM1 homolog, yeast); Exportin-1; exportin-1 (required for chromosome region maintenance)
基因别名: AA420417; CRM1; emb; Exp1; XPO1
UniProt ID: (Human) O14980, (Mouse) Q6P5F9
Entrez Gene ID: (Human) 7514, (Mouse) 103573
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
negative regulation of transcription from RNA polymerase II promoter
protein export from nucleus
sister chromatid cohesion
regulation of centrosome duplication
viral process
protein localization to nucleus
regulation of protein catabolic process
response to drug
regulation of mRNA stability
regulation of protein export from nucleus
mRNA transport
intracellular transport of virus
transport
intracellular protein transport
protein transport
