Invitrogen

CRY2 Polyclonal Antibody

CRY2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CRY2 Antibody (PA5-13125) in IHC (P)

Immunohistochemistry analysis of CRY2 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with CRY2 polyclonal antibody (Product # PA5-13125) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepato... View More

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产品信息

PA5-13125

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:50-1:100

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Ammonium sulfate precipitation, Size-exclusion - Dialysis

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2083988

靶标信息

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Diurnal oscillations of endogenous H₂O₂ sustained by p66^Shc regulate circadian clocks.

Nature cell biology

Pei JF,Li XK,Li WQ,Gao Q,Zhang Y,Wang XM,Fu JQ,Cui SS,Qu JH,Zhao X,Hao DL,Ju D,Liu N,Carroll KS,Yang J,Zhang EE,Cao JM,Chen HZ,Liu DP

PA5-13125 was used in Western Blotting to suggest that redox signalling rhythms are intrinsically coupled to the circadian system through reversible oxidative modification of CLOCK and constitute essential mechanistic timekeeping components in mammals.