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CTCF Antibody (600-401-C42) in IHC (P)
Immunohistochemistry of rabbit anti-CTCF antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-CTCF at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CTCF as precipitated red signal with hematoxylin purple nuclear counterstain.
产品信息
600-401-C42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.
靶标信息
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF
基因别名: AW108038; CTCF; MRD21
UniProt ID: (Human) P49711, (Mouse) Q61164
Entrez Gene ID: (Human) 10664, (Mouse) 13018
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
zinc ion binding
chromatin insulator sequence binding
sequence-specific DNA binding
transcription regulatory region DNA binding
nucleic acid binding
DNA binding
chromatin binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
regulation of gene expression by genetic imprinting
transcription from RNA polymerase II promoter
chromosome segregation
maintenance of DNA methylation
positive regulation of gene expression
covalent chromatin modification
nucleosome positioning
regulation of histone methylation
regulation of histone acetylation
regulation of gene expression, epigenetic
regulation of molecular function, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of centromeric sister chromatid cohesion
transcription, DNA-templated
regulation of transcription, DNA-templated
dosage compensation by inactivation of X chromosome
negative regulation of gene expression
chromatin modification
