Invitrogen

CTRL Polyclonal Antibody

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抗体检测数据 (2)

CTRL Antibody in Western Blot (WB)

CTRL Antibody in Western Blot (WB)

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CTRL Antibody (PA5-143991) in WB

Western blot analysis of CTRL using CTRL antibody (Product # PA5-143991). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human PCP tissue lysates; Lane 2: rat pancreas tissue lysates; Lane 3: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5... View More

CTRL Antibody in Western Blot (WB)

CTRL Antibody in Flow Cytometry (Flow)

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CTRL Antibody in Western Blot (WB) — Western blot analysis of CTRL using CTRL antibody (Product # PA5-143991). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human PCP tissue lysates; Lane 2: rat pancreas tissue lysates; Lane 3: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTRL antigen affinity purified polyclonal antibody (Product # PA5-143991) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTRL at approximately 28 kDa. The expected band size for CTRL is at 28 kDa.

CTRL Antibody in Flow Cytometry (Flow) — Flow cytometry of CTRL in HepG2 cells (Blue line). The cells were blocked with 10% normal goat serum, and then incubated with CTRL polyclonal antibody (Product # PA5-143991) (1 µg/1x10^6 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Unlabeled sample (Red line) was also used as a control. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C.

产品信息

PA5-143991

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

-

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human CTRL recombinant protein (Position: I34-N264).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3075205

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

CTRL is a protein coding gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: chymopasin; chymotrypsin A CTRA-1; chymotrypsin-like; Chymotrypsin-like protease CTRL-1

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基因别名: 0910001G08Rik; 1810004D15Rik; AV005227; chymopasin; Ctra-1; Ctra1; CTRL; CTRL1; mFLJ00366

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UniProt ID: (Human) P40313

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Entrez Gene ID: (Human) 1506, (Rat) 117184, (Mouse) 109660

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serine-type endopeptidase activity peptidase activity serine-type peptidase activity serine protease

参与通路

proteolysis digestion