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CXCL1 Antibody (500-P115-1MG) in WB
Western Blot: To detect Murine KC (CXCL1) by Western Blot analysis CXCL1 Polyclonal Antibody (Product # 500-P115-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine KC (CXCL1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P115-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine KC (CXCL1)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929283
产品详细信息
AA Sequence of recombinant protein: APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1). Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Murine KC (CXCL1) matrix.
Sandwich ELISA: To detect Murine KC (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine KC (CXCL1) (500-P115Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine KC (CXCL1).
Western Blot: To detect Murine KC (CXCL1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine KC (CXCL1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; CXCL-1; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51
基因别名: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1
UniProt ID: (Mouse) P12850
Entrez Gene ID: (Mouse) 14825
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of sodium ion transport
neutrophil chemotaxis
response to lipopolysaccharide
positive regulation of superoxide anion generation
positive regulation of potassium ion transport
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
