Invitrogen

CXCL1 Monoclonal Antibody (48415)

高级验证

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

3个已发表图片

2 篇参考文献

查看其他29个CXCL1 (GRO alpha)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

已发表图片 (3)
高级验证 (1)

CXCL1 Antibody in Immunohistochemistry (IHC)

图: 1 / 4

CXCL1 Antibody (MA5-23745) in IHC

Immunity 2020 - Figure 2 FALCs Are Covered by a Differentiated Monolayer of Mesothelial Cells (A) 3D reconstruction of a large portion of the omentum obtained by imaging of wholemount staining of the omentum showing omFALCs with DAPI (white). (B and C) Confocal imaging and 3D reconstruction of an omFALC showing a view of the surface of the cluster and a z section (along the dotted line) of the cluster (B) and of the surfac... View More

Product Image Gallery

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CXCL1 is shown by the decrease in fluorescence (Chemotaxis measured by Resazurin) with increasing concentrations of CXCL1 Antibody (Product # MA5-23745). {Neu}

产品信息

MA5-23745

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

中和实验 (Neu)

0.05-0.25 µg/mL

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

48415

抗原

E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1 Arg20-Lys96

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2609463

产品详细信息

This antibody shows 100% cross-reactivity with recombinant human (rh) CXCL2, less than 40% cross-reactivity with recombinant rat (rr) CINC-1, and no cross-reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse (rm) CXCL6.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants.

Immunity

Jackson-Jones LH,Smith P,Portman JR,Magalhaes MS,Mylonas KJ,Vermeren MM,Nixon M,Henderson BEP,Dobie R,Vermeren S,Denby L,Henderson NC,Mole DJ,Bénézech C

MA5-23745 was used in Immunohistochemistry-immunofluorescence to show how specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.

Tue Apr 14 00:00:00 EDT 2020

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants.

Immunity

Jackson-Jones LH,Smith P,Portman JR,Magalhaes MS,Mylonas KJ,Vermeren MM,Nixon M,Henderson BEP,Dobie R,Vermeren S,Denby L,Henderson NC,Mole DJ,Bénézech C

Tue Apr 14 00:00:00 EDT 2020

生物信息学

蛋白别名: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; CXCL-1; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

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基因别名: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

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UniProt ID: (Mouse) P12850

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Entrez Gene ID: (Mouse) 14825

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功能

cytokine activity chemokine activity growth factor activity CXCR chemokine receptor binding chemokine

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