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Invitrogen
CXCL1 (GRO alpha) Monoclonal Antibody (KTYFLF), eFluor™ 660, eBioscience™
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CXCL1 (GRO alpha) Antibody (50-7515-42) in Flow
Normal human peripheral blood monocytes were unstimulated (left) or stimulated with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were fixed and stained intracellularly with Anti-Human CD14 PE (Product # 12-0149-42) and Anti-Human CXCL1 eFluor® 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Cells in the monocyt... View More
产品信息
50-7515-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
KTYFLF
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574293
产品详细信息
Description: This KTYFLF monoclonal antibody reacts with human CXCL1 and has no cross-reactivity to human CXCL2 or CXCL3. CXCL1, or GRO (growth-related oncogene) alpha, is a pro-inflammatory CXC chemokine first identified by its constitutive overexpression in some tumors. It is closely related to CXCL2 (GRO beta) and CXCL3 (GRO gamma), with which it shares 90% and 86% sequence homology, respectively. These proteins, along with IL-8 or CXCL8, were later found to be critical for neutrophil mobilization and degranulation, as well as vascular permeabilization and angiogenesis. Signaling occurs through the G protein-coupled receptor CXCR2, which is shared with GRO beta and gamma. CXCR2 can also be activated by IL-8, although some studies suggest that the majority of IL-8 activity on neutrophils is mediated by CXCR1.
CXCL1 is secreted by monocytes, epithelial cells, and fibroblasts in response to pro-inflammatory stimuli such as LPS, IL-1 beta, and TNF alpha. Overexpression is observed in many malignant tumors, where it contributes to tumor vascularization and metastasis.
Applications Reported: This KTYFLF antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This KTYFLF antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of peripheral blood monocytes. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXC; CXC motif chemokine; CXCL; CXCL-1; fibroblast secretory protein; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3
基因别名: CXCL1; FSP; GRO; GRO1; GROA; MGSA; MGSA-a; NAP-3; SCYB1
UniProt ID: (Human) P09341
Entrez Gene ID: (Human) 2919
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
nervous system development
cell proliferation
negative regulation of cell proliferation
actin cytoskeleton organization
response to lipopolysaccharide
intracellular signal transduction
positive regulation of catalytic activity
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil chemotaxis
