Invitrogen

CXCL13 Polyclonal Antibody

高级验证

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

5 篇参考文献

查看其他19个CXCL13抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

CXCL13 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CXCL13 Antibody (PA5-47018) in IHC (F)

Immunohistochemical analysis of CXCL13 in perfusion fixed frozen sections of mouse intestine (Peyers patch). Samples were incubated with CXCL13 polyclonal antibody (Product # PA5-47018) using a dilution of 15 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells.

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产品信息

PA5-47018

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

5-15 µg/mL

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酶联免疫吸附实验 (ELISA)

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中和实验 (Neu)

20-50 µg/mL

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant mouse CXCL13/BLC/BCA-1 Ile22-Ala109

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2610214

产品详细信息

In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt’s Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

IL-10 Promotes CXCL13 Expression in Macrophages Following Foot-and-Mouth Disease Virus Infection.

International journal of molecular sciences

Guo Z,Chen F,Zhao S,Zhang Z,Zhang H,Bai L,Zhang Z,Li Y

PA5-47018 was used in Enzyme-linked immunosorbent assay to demonstrate for the first time that macrophages and T cells were the cellular sources of CXCL13 in mice infected with FMDV; CXCL13 was produced in BMDMs via NF-κB and JAK/STAT pathways; and IL-10 promoted CXCL13 expression in BMDMs via the JAK/STAT pathway.

Tue Mar 28 00:00:00 EDT 2023

IL-10 Promotes CXCL13 Expression in Macrophages Following Foot-and-Mouth Disease Virus Infection.

International journal of molecular sciences

Guo Z,Chen F,Zhao S,Zhang Z,Zhang H,Bai L,Zhang Z,Li Y

Tue Mar 28 00:00:00 EDT 2023

Lymph node formation and B cell homeostasis require IKK-α in distinct endothelial cell-derived compartments.

Proceedings of the National Academy of Sciences of the United States of America

McCorkell KA,Jayachandran N,Cully MD,Freund-Brown J,Weinkopff T,Monslow J,Hu Y,Puré E,Freedman BD,Alvarez JI,Cancro MP,May MJ

PA5-47018 was used in Immunohistochemistry-immunofluorescence to reveal that IKK-α in distinct EC-derived compartments is uniquely required to promote B cell homeostasis and LN development, and establish that LEC-intrinsic IKK-α is absolutely essential for LN formation.

Tue Nov 30 00:00:00 EST 2021

CXCR5/NRF2 double knockout mice develop retinal degeneration phenotype at early adult age.

Experimental eye research

Huang H,Lennikov A

PA5-47018 was used in Immunohistochemistry (Paraffin) to characterize the retinal degeneration (RD) phenotype of CXCR5/NRF2 double knockout (DKO) mice at the early adult age.

Wed Jul 01 00:00:00 EDT 2020

Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation.

Frontiers in immunology

Lennikov A,Saddala MS,Mukwaya A,Tang S,Huang H

PA5-47018 was used in Western Blotting to show that with age, CXCR5 mice develop retinal degeneration characterised by microglia dysfunction, increased production of CXCL13 in the RPE progressive photoreceptor and neuronal loss.

Mon Oct 05 00:00:00 EDT 2020

生物信息学

蛋白别名: B lymphocyte chemoattractant; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 13; CC motif chemokine; CCmotif chemokine; chem; CXC; CXC chemokine BLC; CXC motif chemokine; CXC motif chemokine 13; CXCL; small inducible cytokine subfamily B (Cys-X-Cys), member 13; Small-inducible cytokine B13

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基因别名: 4631412M08Rik; Angie; ANGIE2; BCA-1; BLC; BLR1L; Cxcl13; Scyb13

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UniProt ID: (Mouse) O55038

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Entrez Gene ID: (Mouse) 55985

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功能

cytokine activity chemokine activity heparin binding fibroblast growth factor binding CXCR5 chemokine receptor binding CCR10 chemokine receptor binding protein heterodimerization activity receptor agonist activity CXCR3 chemokine receptor binding chemokine

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