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AA Sequence of recombinant protein: AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography employing an immobilized Human ENA-78 (CXCL5) matrix.
Sandwich ELISA: To detect Human ENA-78 (CXCL5) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ENA-78 (500-P91Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human ENA-78 (CXCL5).
Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 5; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; CXC; CXC motif chemokine; CXCL; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); Small-inducible cytokine B5
基因别名: CXCL5; ENA-78; ENA78; SCYB5
UniProt ID: (Human) P42830
Entrez Gene ID: (Human) 6374
分子生物学功能: chemokine