Invitrogen

CXCL9 Recombinant Rabbit Monoclonal Antibody (11H1L14)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

5 篇参考文献

查看其他27个CXCL9 (MIG)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CXCL9 Antibody in Immunocytochemistry (ICC/IF)

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CXCL9 Antibody (701117) in ICC/IF

Immunofluorescence analysis of CXCL9/MIG was performed using 70% confluent log phase THP-1 cells treated with 100 ng of IFN-gamma for 15 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CXCL9/MIG (11H1L14) Recombinant Rabbit Monoclonal Antibody (Product # 701117) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Cha... View More

CXCL9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CXCL9 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCL9 Antibody — Modulation of expression of target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of CXCL9 using anti-CXCL9 rabbit monoclonal antibody (Product # 701117) shows increase in expression of CXCL9 in theTHP1 cell upon treatment with IFNgamma in cytoplasm {TM}

产品信息

701117

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

2 µg/mL

点杂交 (DB)

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

11H1L14

抗原

Recombinant protein corresponding to amino acids 23-125 of human monokine induced by Interferon-gamma

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532396

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma.

EBioMedicine

Wen T,Sun G,Jiang W,He X,Shi Y,Ma F,Liu P

701117 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the combined effect of the humanized PD1 antibody and different HDAC inhibitors in human PD1 gene knock-in mouse models, illustrate the underlying mechanisms, and discover predictive biomarkers.

Sun Jan 01 00:00:00 EST 2023

Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma.

EBioMedicine

Wen T,Sun G,Jiang W,He X,Shi Y,Ma F,Liu P

Sun Jan 01 00:00:00 EST 2023

Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.

The Journal of clinical investigation

Cai W,Shi L,Zhao J,Xu F,Dufort C,Ye Q,Yang T,Dai X,Lyu J,Jin C,Pu H,Yu F,Hassan S,Sun Z,Zhang W,Hitchens TK,Shi Y,Thomson AW,Leak RK,Hu X,Chen J

701117 was used in Immunohistochemistry to show that these unique CD8+ TRLs serve as early responders to rally defenses against stroke, offering fresh perspectives for clinical translation.

Mon Aug 01 00:00:00 EDT 2022

Senescent hepatocytes enhance natural killer cell activity via the CXCL-10/CXCR3 axis.

Experimental and therapeutic medicine

Zang J,Ye J,Zhang C,Sha M,Gao J

Published figure using CXCL9 recombinant monoclonal antibody (Product # 701117) in Dot blot

Fri Nov 01 00:00:00 EDT 2019

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Nature communications

Pascual-García M,Bonfill-Teixidor E,Planas-Rigol E,Rubio-Perez C,Iurlaro R,Arias A,Cuartas I,Sala-Hojman A,Escudero L,Martínez-Ricarte F,Huber-Ruano I,Nuciforo P,Pedrosa L,Marques C,Braña I,Garralda E,Vieito M,Squatrito M,Pineda E,Graus F,Espejo C,Sahuquillo J,Tabernero J,Seoane J

701117 was used in Immunohistochemistry to study the effect of the combination of LIF neutralising antibodies with the inhibition of the PD1 immune checkpoint on tumour progression.

Tue Jun 11 00:00:00 EDT 2019

生物信息学

蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 9; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 9; CXC; CXC motif chemokine; CXCL; gamma interferon-induced monokine; Gamma-interferon-induced monokine; HuMIG; M119; MIG; monokine induced by gamma interferon; Monokine induced by interferon-gamma; Protein m119; small inducible cytokine B subfamily (Cys-X-Cys), member 9; Small-inducible cytokine B9

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基因别名: BB139920; CMK; crg-10; CXCL9; Humig; MIG; MuMIG; SCYB9

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UniProt ID: (Human) Q07325, (Mouse) P18340

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Entrez Gene ID: (Human) 4283, (Mouse) 17329

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功能

cytokine activity protein binding chemokine activity CXCR3 chemokine receptor binding chemokine

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