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CYB5R3 Antibody (MA5-44799) in WB
Western blot analysis of CYB5R3 in different lysates. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in CYB5R3 Monoclonal antibody (Product # MA5-44799) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Lane 1: PC-12 cell lysate (10 µg/Lane); Lane 2: Rat lung tissue lysate (20... View More
产品信息
MA5-44799
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE63-51
抗原
Recombinant protein within human CYB5R3 aa 1-150/301.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2931256
靶标信息
CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B5R; Diaphorase (NADH) (cytochrome b-5 reductase); diaphorase 1; Diaphorase-1; Diaphorase1; mutant NADH-cytochrome b5 reductase; NADH-cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form
基因别名: B5R; CYB5R3; DIA1; Nadhcb5; RNNADHCB5
UniProt ID: (Human) P00387, (Rat) P20070
Entrez Gene ID: (Human) 1727, (Rat) 25035
