Invitrogen

CYBA Monoclonal Antibody (CS9)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他7个CYBA抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (1)

CYBA Antibody in Immunocytochemistry (ICC/IF)

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CYBA Antibody (MA5-18047) in ICC/IF

Immunofluorescence analysis of CYBA was performed using 70% confluent log phase PC-3, DU 145 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CYBA Monoclonal Antibody (CS9) (Product # MA5-18047) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More

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CYBA Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CYBA Monoclonal Antibody (CS9) (Product # MA5-18047), shows punctate nuclear staining in PC-3 and DU 145, prostate cancerous cell lines with high ROS levels, in comparison to BJ, a normal fibroblast cell line, with low endogenous ROS. {RE}

产品信息

MA5-18047

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CS9

抗原

Partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in octyl glucoside)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539430

产品详细信息

This antibody is specific for human Cytochrome b245 light chain (aa 165-169, KKPSE).

This target has a predicted molecular weight of 21 kDa.

靶标信息

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: cytochrome b light chain; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; Cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit

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基因别名: CYBA; p22-PHOX

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UniProt ID: (Human) P13498

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Entrez Gene ID: (Human) 1535

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功能

protein binding electron carrier activity superoxide-generating NADPH oxidase activity SH3 domain binding heme binding metal ion binding protein heterodimerization activity

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