Invitrogen

CYLD Monoclonal Antibody (733)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

3 篇参考文献

查看其他16个CYLD抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CYLD Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

CYLD Antibody (43-7700) in ICC/IF

Immunofluorescence analysis of CYLD was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with CYLD Mouse Monoclonal Antibody (Product # 43-7700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution... View More

CYLD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CYLD Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with CYLD siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CYLD Monoclonal Antibody (733) (Product # 43-7700). {KD}

产品信息

43-7700

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:50

免疫细胞化学 (ICC/IF)

1 µg/mL

产品规格

种属反应

Bovine, Horse, Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

733

抗原

Recombinant protein derived from the N-terminus of human CYLD protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533539

靶标信息

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.

Current biology : CB

Hotta T,McAlear TS,Yue Y,Higaki T,Haynes SE,Nesvizhskii AI,Sept D,Verhey KJ,Bechstedt S,Ohi R

43-7700 was used in Western Blot to increase microtubule stability.

Mon Sep 26 00:00:00 EDT 2022

Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1 (HSV-1) protein ICP6 triggers host antiviral defense.

Proceedings of the National Academy of Sciences of the United States of America

Wang X,Li Y,Liu S,Yu X,Li L,Shi C,He W,Li J,Xu L,Hu Z,Yu L,Yang Z,Chen Q,Ge L,Zhang Z,Zhou B,Jiang X,Chen S,He S

Published figure using CYLD monoclonal antibody (Product # 43-7700) in Western Blot

Tue Oct 28 00:00:00 EDT 2014

Necroptosis mediates TNF-induced toxicity of hippocampal neurons.

BioMed research international

Liu S,Wang X,Li Y,Xu L,Yu X,Ge L,Li J,Zhu Y,He S

Published figure using CYLD monoclonal antibody (Product # 43-7700) in Western Blot

Mon Mar 30 00:00:00 EDT 2015

生物信息学

蛋白别名: cylindromatosis (turban tumor syndrome); Deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; retinitis pigmentosa 1 homolog; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD

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基因别名: 2010013M14Rik; 2900009M21Rik; BRSS; C130039D01Rik; CDMT; CYLD; CYLD1; CYLDI; EAC; HSPC057; KIAA0849; LRRGT00003; MFT; MFT1; mKIAA0849; Rp1; Rp1h; SBS; TEM; USPL2