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CYP1B1 Antibody (PA5-95277) in IHC (P)
Immunohistochemistry analysis of CYP1B1 in paraffin-embedded human liver cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with CYP1B1 polyclonal antibody (Product # PA5-95277) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex... View More
产品信息
PA5-95277
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human CYP1B1 recombinant protein (Position: R255-L480).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2807081
产品详细信息
Human CYP1B1 shares 85.4% and 84.5% amino acid (aa) sequence identity with mouse and rat CYP1B1, respectively.^M
^M
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
CYP1B1 is a 543 amino acid intracellular protein localized to the endoplasmic reticulum. It belongs to the cytochrome P450 super family of enzymes which are monooxygenases and it catalyzes many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol and is also involved in the metabolism of substances active in the eye growth and differentiation and hence plays a putative role in ocular development. Mutated proteins are known to cause primary congenital glaucoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: aryl hydrocarbon hydroxylase; benz[a]anthracene inducible; CYPIB1; Cytochrome P450 1B1; cytochrome P450, 1b1, benz[a]anthracene inducible; cytochrome P450, family 1, subfamily B, polypeptide 1; cytochrome P450, subfamily 1B, polypeptide 1; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile); Cytochrome P450CMEF; Cytochrome P450EF; Cytochrome P450RAP; flavoprotein-linked monooxygenase; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P450RAP; xenobiotic monooxygenase
基因别名: CP1B; Cyp1-b1; CYP1B1; CYPIB1; GLC3A; P4501B1
UniProt ID: (Human) Q16678, (Rat) Q64678, (Mouse) Q64429
Entrez Gene ID: (Human) 1545, (Rat) 25426, (Mouse) 13078
monooxygenase activity
iron ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
heme binding
aromatase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
metal ion binding
oxidoreductase
oxygenase
metabolite interconversion enzyme
angiogenesis
trabecular meshwork development
cellular aromatic compound metabolic process
xenobiotic metabolic process
nitric oxide biosynthetic process
cell adhesion
visual perception
steroid metabolic process
estrogen metabolic process
negative regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
toxin metabolic process
response to toxic substance
positive regulation of vascular endothelial growth factor production
sterol metabolic process
arachidonic acid metabolic process
epoxygenase P450 pathway
collagen fibril organization
negative regulation of cell migration
negative regulation of NF-kappaB transcription factor activity
negative regulation of cell adhesion mediated by integrin
retinol metabolic process
retinal metabolic process
positive regulation of apoptotic process
endothelial cell migration
positive regulation of angiogenesis
positive regulation of JAK-STAT cascade
membrane lipid catabolic process
blood vessel morphogenesis
oxidation-reduction process
retinal blood vessel morphogenesis
cellular response to hydrogen peroxide
cellular response to organic cyclic compound
endothelial cell-cell adhesion
omega-hydroxylase P450 pathway
regulation of reactive oxygen species metabolic process
DNA modification
response to nutrient
male gonad development
response to organic substance
response to organic cyclic compound
positive regulation of smooth muscle cell migration
dibenzo-p-dioxin metabolic process
adrenal gland development
response to follicle-stimulating hormone
response to estradiol
benzene-containing compound metabolic process
estrous cycle
positive regulation of translation
response to arsenic-containing substance
response to steroid hormone
retina vasculature development in camera-type eye
ganglion development
cellular response to cAMP
cellular response to tumor necrosis factor
cellular response to luteinizing hormone stimulus
cellular response to cortisol stimulus
cellular response to progesterone stimulus
response to dexamethasone
response to indole-3-methanol
positive regulation of reactive oxygen species metabolic process
positive regulation of DNA biosynthetic process
