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Invitrogen

CYR61 Polyclonal Antibody

1 Published Figure
2 References
View all (11) CYR61 antibodies
Datasheet
Protocols
Tech Support
Datasheet
Protocols
Tech Support

Product Details

PA5-78022

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYR61. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735700

Product Specific Information

Positive Control: HeLa (1X Brefeldin A for 24hr)

Predicted Reactivity: Chicken (81%), Chimpanzee (99%), Bovine (92%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CYR61 is a secreted heparin binding protein, encoded by a growth factor inducible immediate-early gene that associates with the extracellular matrix and connective tissue. CYR61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins, which includes connective tissue growth factor (CTGF) and the mouse CYR61 homolog, Fisp12. As an angiogenic inducer, CYR61 binds to the cell surface receptor integrin alphavbeta3, where it then stimulates cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Expression of CYR61 is elevated during vessel growth, wound healing and chondrocyte differentiation. CYR61 is also detected in a wide variety of tumors as it induces tumor growth and functions as a marker of tumor progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3CH61; CCN family member 1; Cellular communication network factor 1; cysteine rich protein 61; Cysteine-rich angiogenic inducer 61; cysteine-rich heparin-binding protein 61; cysteine-rich, angiogenic inducer, 61; cysteine-rich, anigogenic inducer, 61; IBP-10; IGF-binding protein 10; IGFBP-10; insulin-like growth factor binding protein 10; Insulin-like growth factor-binding protein 10; intermediate early; Protein CYR61; Protein GIG1

Gene Aliases: AI325051; CCN1; CYR61; GIG1; IGFBP10

UniProt ID: (Human) O00622, (Rat) Q9ES72, (Mouse) P18406

Entrez Gene ID: (Human) 3491, (Rat) 83476, (Mouse) 16007

Function(s)
integrin binding insulin-like growth factor binding heparin binding extracellular matrix binding growth factor binding growth factor
Process(es)
regulation of cell growth osteoblast differentiation positive regulation of protein phosphorylation intussusceptive angiogenesis atrioventricular valve morphogenesis apoptotic process involved in heart morphogenesis ventricular septum development chemotaxis cell adhesion cell-cell signaling cell proliferation anatomical structure morphogenesis positive regulation of phospholipase activity positive regulation of cell-substrate adhesion extracellular matrix organization positive regulation of cell migration positive regulation of BMP signaling pathway positive regulation of osteoblast proliferation negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process wound healing, spreading of cells positive regulation of osteoblast differentiation positive regulation of protein kinase activity positive regulation of transcription from RNA polymerase II promoter atrial septum morphogenesis negative regulation of cell death chondroblast differentiation chorio-allantoic fusion labyrinthine layer blood vessel development positive regulation of cartilage development regulation of ERK1 and ERK2 cascade reactive oxygen species metabolic process positive regulation of ceramide biosynthetic process signal transduction single organismal cell-cell adhesion positive regulation of apoptotic process positive regulation of cell differentiation positive regulation of angiogenesis
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