Invitrogen

CaMKIV Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 篇参考文献

查看其他25个CaMKIV抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (7)
高级验证 (1)

CaMKIV Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

CaMKIV Antibody (PA1-542) in ICC/IF

Immunofluorescence analysis of CaM Kinase IV was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CaM Kinase IV Rabbit Polyclonal Antibody (Product # PA1-542) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More

CaMKIV Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA1-542

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:100

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Plant, Rat, Xenopus

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: V(127) E K G Y Y S E R D A A D A V K Q(143) C

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325834

产品详细信息

PA1-542 detects CaM kinase IV (CaMKIV) from human, mouse, rat, canine, primate, arabidopsis and Xenopus Oocyte samples.

PA1-542 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.

PA1-542 immunizing peptide corresponds to amino acid residues 127-143 from mouse CaMKIV. This sequence is 94% conserved in rat and human CaMKIV. This peptide (Cat. PEP-109) is available for use in neutralization and control experiments.

靶标信息

CAMK4 (CaMKIV) is a serine/threonine protein kinase that phosphoryates CREB, oncoprotein 18, and SRF. It has limited tissue distribution, and has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease.

The Journal of clinical investigation

Maeda K,Otomo K,Yoshida N,Abu-Asab MS,Ichinose K,Nishino T,Kono M,Ferretti A,Bhargava R,Maruyama S,Bickerton S,Fahmy TM,Tsokos MG,Tsokos GC

PA1-542 was used in Immunofluorescence to explore the role of Ca2+/calmodulin-dependent kinase signalling in podocytes.

Wed Aug 01 00:00:00 EDT 2018

A unique de novo gain-of-function variant in CAMK4 associated with intellectual disability and hyperkinetic movement disorder.

Cold Spring Harbor molecular case studies

Zech M,Lam DD,Weber S,Berutti R,Poláková K,Havránková P,Fečíková A,Strom TM,Růžička E,Jech R,Winkelmann J

PA1-542 was used in Western Blotting to study the importance of CAMK4 in human neurodevelopment, provide a foundation for future clinical research of CAMK4, and suggest the CaMKIV signaling pathway as a potential drug target in neurological disease.

Sat Dec 01 00:00:00 EST 2018

Potential role of cAMP response element-binding protein in ethanol-induced N-methyl-D-aspartate receptor 2B subunit gene transcription in fetal mouse cortical cells.

Molecular pharmacology

Rani CS,Qiang M,Ticku MK

PA1-542 was used in western blot to investigate the intracellular signaling pathways related to NR2B gene transcription regulated by long-term ethanol treatment

Wed Jun 01 00:00:00 EDT 2005

生物信息学

蛋白别名: brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; Ca2+/calmodulin-dependent protein kinase type IV/Gr; Calcium/calmoduli; calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calmodulin-dependent protein kinase IV; Calspermin; CAM kinase IV; CAM kinase- GR; CAM kinase-GR; CaMK IV; EC 2.7.11.17; KCC4; kinase CaMK4; OTTHUMP00000222818

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基因别名: A430110E23Rik; AI666733; CAMK; CaMK IV; CAMK-GR; CAMK4; camk4-A; CAMKIV; CaMKIV/Gr; Ccdpk; D18Bwg0362e; RATCCDPK; xCaM; XELAEV_18008011mg

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UniProt ID: (Human) Q16566, (Rat) P13234