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Invitrogen
CaMKIV Polyclonal Antibody
Application
FIGURE: 1 / 8
CaMKIV Antibody (PA1-542) in ICC/IF
Immunofluorescence analysis of CaM Kinase IV was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CaM Kinase IV Rabbit Polyclonal Antibody (Product # PA1-542) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
PA1-542
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Plant,
Rat,
Xenopus
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: V(127) E K G Y Y S E R D A A D A V K Q(143) C
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325834
Product Specific Information
PA1-542 detects CaM kinase IV (CaMKIV) from human, mouse, rat, canine, primate, arabidopsis and Xenopus Oocyte samples.
PA1-542 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.
PA1-542 immunizing peptide corresponds to amino acid residues 127-143 from mouse CaMKIV. This sequence is 94% conserved in rat and human CaMKIV. This peptide (Cat. PEP-109) is available for use in neutralization and control experiments.
Target Information
CAMK4 (CaMKIV) is a serine/threonine protein kinase that phosphoryates CREB, oncoprotein 18, and SRF. It has limited tissue distribution, and has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; Ca2+/calmodulin-dependent protein kinase type IV/Gr; Calcium/calmoduli; calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calmodulin-dependent protein kinase IV; Calspermin; CAM kinase IV; CAM kinase- GR; CAM kinase-GR; CaMK IV; EC 2.7.11.17; KCC4; kinase CaMK4; OTTHUMP00000222818
Gene Aliases: A430110E23Rik; AI666733; CAMK; CaMK IV; CAMK-GR; CAMK4; camk4-A; CAMKIV; CaMKIV/Gr; Ccdpk; D18Bwg0362e; RATCCDPK; xCaM; XELAEV_18008011mg
UniProt ID: (Human) Q16566, (Rat) P13234
Entrez Gene ID: (Human) 814, (Dog) 100684140, (Mouse) 12326, (Rat) 25050, (Xenopus) 373837
calmodulin-dependent protein kinase activity
calmodulin binding
ATP binding
calcium-dependent protein serine/threonine kinase activity
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
adaptive immune response
myeloid dendritic cell cytokine production
protein phosphorylation
inflammatory response
signal transduction
long-term memory
peptidyl-serine phosphorylation
regulation of T cell differentiation in thymus
intracellular signal transduction
myeloid dendritic cell differentiation
regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
protein autophosphorylation
immune system process
nucleocytoplasmic transport
neuron-neuron synaptic transmission
phosphorylation
positive regulation of protein export from nucleus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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