Invitrogen

Calmodulin Recombinant Rabbit Monoclonal Antibody (SJ16-09)

Calmodulin Antibody in Immunocytochemistry (ICC/IF)

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Calmodulin Antibody (MA5-32074) in ICC/IF

Immunocytochemical analysis of Pan-Calmodulin in MCF-7 cells using a Pan-Calmodulin Monoclonal antibody (Product # MA5-32074) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Calmodulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calmodulin Antibody in Western Blot (WB)

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产品信息

MA5-32074

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SJ16-09

抗原

Synthetic peptide within Human Calmodulin aa 100-149

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809368

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.

Cell systems

Hark TJ,Rao NR,Castillon C,Basta T,Smukowski S,Bao H,Upadhyay A,Bomba-Warczak E,Nomura T,O'Toole ET,Morgan GP,Ali L,Saito T,Guillermier C,Saido TC,Steinhauser ML,Stowell MHB,Chapman ER,Contractor A,Savas JN

MA5-32074 was used in immunohistochemistry (frozen) to observe protein turnover at early stages of amyloid beta (Aβ) proteotoxicity.

Wed Feb 17 00:00:00 EST 2021

生物信息学

蛋白别名: calcium binding gene, DD112; Calcium-binding protein Dd112; CALM 1; calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); calmodulin 3 (phosphorylase kinase, delta); calmodulin 5; Calmodulin III; Calmodulin-1; Calmodulin-2; Calmodulin-3; Calmodulin-4; caM; CAMI; DD312; epididymis secretory protein Li 72; LP7057 protein; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3; skin calmodulin-related factor

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基因别名: 1500001E21Rik; 2310037J09Rik; AI256814; AI327027; AI461935; AI649108; AL024000; AL024017; AY314008; CALM; CALM1; CALM2; CALM3; Calm4; CALML2; CAM; CAM1; CAM2; CAM3; CaMA; CAMB; CAMC; CAMI; CAMII; CAMIII; CPVT4; DD112; DD132; HEL-S-72; LQT14; LQT15; PHKD; PHKD2; PHKD3; R75142; RNCAMIII; Scarf; Scarf2