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Calpastatin Antibody (MA5-16327) in IHC (P)
Immunohistochemical analysis of Calpastatin using anti-Calpastatin Monoclonal Antibody (Product # MA5-16327) in Breast Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
产品信息
MA5-16327
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
SP82
抗原
Synthetic peptide derived from near C-terminus of human calpastatin protein.
偶联物
Unconjugated
形式
Liquid
纯化类型
Protein A/G
保存液
PBS, pH 7.6, with 1% BSA
内含物
0.1% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537846
产品详细信息
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast ductal carcinoma.
靶标信息
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Calpain inhibitor; Calpastatin; Sperm BS-17 component
基因别名: BS-17; CAST; PLACK
UniProt ID: (Human) Q96D08
Entrez Gene ID: (Human) 831
