Invitrogen

Calprotectin Monoclonal Antibody (MAC387)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 篇参考文献

查看其他37个Calprotectin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (3)

Calprotectin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

Calprotectin Antibody (MA1-33972) in ICC/IF

Immunofluorescence analysis of Calprotectin was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin Monoclonal Antibody (MAC387) (Product # MA1-33972) at 1:250 dilution in 0.1% BSA, incubated at 4 degr... View More

Calprotectin Antibody in Western Blot (WB)

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产品信息

MA1-33972

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:100-1:200

免疫组化（冰冻） (IHC (F))

1:100-1:200

免疫细胞化学 (ICC/IF)

1:250

流式细胞分析 (Flow)

1:50-1:100

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产品规格

种属反应

Baboon, Bovine, Dog, Horse, Cat, Guinea pig, Goat, Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

MAC387

抗原

Human monocytes

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1957578

产品详细信息

For staining of paraffin sections, protein digestion pre-treatment (e.g. trypsin), 0.1% for 10 minutes or antigen retrieval using heat treatment is required.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Insulin increases resistance to burn wound infection-associated sepsis.

Critical care medicine

Gauglitz GG,Toliver-Kinsky TE,Williams FN,Song J,Cui W,Herndon DN,Jeschke MG

MA1-33972 was used in flow cytometry to investigate the therapeutic efficacy of insulin on the treatment of sepsis

Fri Jan 01 00:00:00 EST 2010

生物信息学

蛋白别名: AI323541; B8Ag; BEE11; BEE22; calcium binding protein; S100 protein; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Chemotactic cytokine CP-10; chemotactic S100 protein; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A

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基因别名: 60B8AG; AI323541; AW546964; B8Ag; BEE22; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; GAGB; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP-8; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9