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Calretinin Antibody (MA5-16328) in IHC (P)
Immunohistochemical (Paraffin) analysis of Calretinin using anti-Calretinin Monoclonal Antibody (Product # MA5-16328) in human mesothelioma at a dilution of 1:100. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated for 15 mins at room temperature and detected using an HRP conjugated c... View More
产品信息
MA5-16328
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
SP13
抗原
Recombinant full-length mouse calretinin protein
偶联物
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
纯化类型
Protein A
保存液
PBS, pH 7.20, with 1% BSA
内含物
0.1% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537847
产品详细信息
This antibody is predicted to react with bovine, chicken and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.
靶标信息
Calretinin is encoded by the CALB2 gene located on chromosome 16. It is a troponin C superfamily protein having six EF-hand structural motifs. The EF motif allows the protein to bind calcium, and function in calcium signaling, calcium buffering, and modulating neuronal excitability. Calretinin is expressed in the mesothelium, mast cells, some neural cells, hair follicles, and fat cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 29 kDa calbindin; 29kD calretinin; 29kDa calbindin; calbindin 2, (29kD, calretinin); calbindin D29K; Calretinin; CR; testicular secretory protein Li 8
基因别名: CAB29; CAL2; CALB2; CR
UniProt ID: (Human) P22676, (Rat) P47728, (Mouse) Q08331
Entrez Gene ID: (Human) 794, (Rat) 117059, (Mouse) 12308
