Invitrogen

Carbonic Anhydrase IV Polyclonal Antibody

Carbonic Anhydrase IV Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Carbonic Anhydrase IV Antibody (PA5-47312) in IHC (F)

Immunohistochemical analysis of Carbonic Anhydrase IV in perfusion fixed frozen sections of mouse kidney. Samples were incubated in Carbonic Anhydrase IV polyclonal antibody (Product # PA5-47312) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytopl... View More

Carbonic Anhydrase IV Antibody in Western Blot (WB)

Carbonic Anhydrase IV Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-47312

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-12.5 µg/mL

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

5-15 µg/mL

免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

25 µg/mL

产品规格

种属反应

Mouse

已发表种属

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4 Glu18-Ser277

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2607329

产品详细信息

In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) CA4 is observed and less than 2% cross-reactivity with rhCA3, rhCA10, rhCA13, recombinant mouse (rm) CA9, and rmCA12 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport.

仅用于科研。不用于诊断过程。未经明确授权不得转售。